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Open Access Highly Accessed Correspondence

T cells fail to develop in the human skin-cell explants system; an inconvenient truth

Bob Meek1, Catharina HMJ Van Elssen12, Mirelle JAJ Huijskens1, Sjoukje JC van der Stegen1, Siebe Tonnaer2, Stijn BJ Lumeij2, Joris Vanderlocht1, Mark A Kirkland3, Reinout Hesselink2, Wilfred TV Germeraad1* and Gerard MJ Bos1

Author Affiliations

1 Department of Internal Medicine, Division of Hematology, Maastricht University Medical Center+, Maastricht, the Netherlands

2 PharmaCell BV, BioPartner Building, Maastricht, the Netherlands

3 Department of Clinical and Biomedical Sciences, Geelong, University of Melbourne, Australia

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BMC Immunology 2011, 12:17  doi:10.1186/1471-2172-12-17

The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1471-2172/12/17


Received:17 September 2010
Accepted:18 February 2011
Published:18 February 2011

© 2011 Meek et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

Haplo-identical hematopoietic stem cell (HSC) transplantation is very successful in eradicating haematological tumours, but the long post-transplant T-lymphopenic phase is responsible for high morbidity and mortality rates. Clark et al. have described a skin-explant system capable of producing host-tolerant donor-HSC derived T-cells. Because this T-cell production platform has the potential to replenish the T-cell levels following transplantation, we set out to validate the skin-explant system.

Results

Following the published procedures, while using the same commercial components, it was impossible to reproduce the skin-explant conditions required for HSC differentiation towards mature T-cells. The keratinocyte maturation procedure resulted in fragile cells with minimum expression of delta-like ligand (DLL). In most experiments the generated cells failed to adhere to carriers or were quickly outcompeted by fibroblasts. Consequently it was not possible to reproduce cell-culture conditions required for HSC differentiation into functional T-cells. Using cell-lines over-expressing DLL, we showed that the antibodies used by Clark et al. were unable to detect native DLL, but instead stained 7AAD+ cells. Therefore, it is unlikely that the observed T-lineage commitment from HSC is mediated by DLL expressed on keratinocytes. In addition, we did confirm expression of the Notch-ligand Jagged-1 by keratinocytes.

Conclusions

Currently, and unfortunately, it remains difficult to explain the development or growth of T-cells described by Clark et al., but for the fate of patients suffering from lymphopenia it is essential to both reproduce and understand how these co-cultures really "work". Fortunately, alternative procedures to speed-up T-cell reconstitution are being established and validated and may become available for patients in the near future.

Background

Lymphopenia results in high mortality and morbidity among cancer patients receiving a hematopoietic stem cell (HSC) transplantation, or suffering from HIV infection [1-5]. Eradication of hematological cancers is very successful using haplo-identical HSC transplantation [6], but many patients succumb to opportunistic infections that are the direct consequence of the lymphopenia, mainly involving the T cell pool [7]; it often takes more than 200 days before (mainly CD4+) T cell levels have normalized again. This underlines the need for, and explains the general interest in, methods capable of enhancing T cell reconstitution [8,9].

Two important problems associated with slow recovery of T cell levels involve the thymus: slow thymic reconstitution by blood-borne progenitors and thymic involution [10-14]. Because it is still not possible to control and/or reverse either of these processes, there is an obvious need to establish methods that generate a de novo T cell repertoire in vitro. However, the development of such systems is hampered because most processes that occur in the thymus are still enigmatic, especially how the thymus is capable to enforce self versus non-self recognition on developing thymocytes [15]. Understanding the process of positive/negative selection, and reproducing the process in vitro, would potentially help to reduce lymphopenia, especially in older patients as the thymus involutes with age. In this context, the results on thymus-independent T cell development previously described by Clark et al. are remarkable. This method involves a seemingly simple co-culture system consisting of skin keratinocytes and fibroblasts grown on a three-dimensional (3D) tantalum covered scaffold (Statamatrix®) that, after 4 weeks co-culture with allogenic HSC, results in a population containing 3-5% T cells tolerant to the skin-donor [16]. Even though T cells were detected, only a limited fraction of the expanded HSC actually became T-lineage committed; many cells differentiated towards Class II+ APC, a convenient aspect since it was suggested to be important for CD4+ T cell development [16]. Additional explanations for the extra-thymic development of T cells were the co-incidental expression of various components known to be important for HSC differentiation and thymus-function, such as Delta-Like Ligand (DLL) [8] and AutoImmune Regulator (AIRE) [17] by keratinocytes and fibroblasts [16], respectively.

Even though direct mechanistic explanations for the extra-thymic development of T cells are lacking, and despite limited numbers, it still remains the only published method potentially capable of generating functional, clinical-grade, mature T cells ex vivo. Because of the clinical importance, we made an effort to establish the skin-cell system in our laboratory and to characterize it in more detail. We observed that keratinocytes do express the Notch ligand Jagged1, but we did not find the abundant expression of DLL protein previously reported by Clark et al. on keratinocytes. Furthermore, due to the growth characteristics of keratinocytes and fibroblasts in the Statamatrix®, we could not reproduce the co-cultures as described by Clark et al. [16]. As a result, we never observed any T-lineage differentiation. The various reasons for the unsuccessful reproduction of this method are described in this report.

Results

The in vitro T cell development from donor CD34+ cells as described by Clark et al. [16] requires a co-culture of (recipient = patient) keratinocytes and fibroblasts. To confirm expression of determinants reported to be important for in vitro differentiation, and establish co-cultures, we prepared keratinocyte and fibroblast cell banks from various donors and different skin sources. Sufficient numbers of early passage stocks were available from 5 donors for all experiments.

Is DLL expressed by keratinocytes?

Many papers have described the necessity for Notch signaling in T cell determination and differentiation from CD34+ cells (reviewed in [8]). One of the most obvious and logical explanations provided by Clark et al. [16] for the generation of T cells was the innate potential of matured keratinocytes to express DLL protein. To detect/confirm DLL expression on intact keratinocytes, we used three different methods.

FACS analysis

In the paper by Clark et al. [16], it was not specified at what stage the keratinocytes were harvested, and which DLL was detected by the polyclonal H265 antibody. In our hands, the differentiation procedure resulted in a mixture of undifferentiated and early-differentiated cells; none of the "differentiated" keratinocytes ever lost CK14 expression, which is a marker for mature keratinocytes (Figure 1). By using murine cell-lines (genetically) over-expressing human DLL1 or 4 it became clear that the H265 antibody used for FACS and IF does recognize DLL1, but not DLL4 (Figure 2A). Using a murine stromal cell line transduced with human DLL1 as positive control, which shows intense staining of DLL1 on immunoblot (Figure 2A), we found that it was possible to detect DLL1 on vital cells by flowcytometry (FACS), albeit with difficulty (Figure 2B). When matured keratinocytes were indirectly stained with H265 for analysis by FACS, we were never able to detect a specific signal from H265 on vital, 7AAD- keratinocytes (Figure 3A). Other primary rabbit antibodies specific for intracellular antigens, normally not expressed by keratinocytes like NOS2 and IRF1, also stained 7AAD- keratinocytes (Figure 3A), indicating that these antibody-preparations may not be suitable for analysis by FACS. We did find that 7AAD+/DAPI+ cells were intensely stained by all primary rabbit antibodies, including H265 (Figure 3B), generating a false positive H265/SSC picture very similar as published by Clark et al. [16]. Apparently, it is possible to use the FACS procedure in combination with H265 to detect DLL1 expression on the surface of DLL1-transfected cell-lines, but it cannot be used to reliably stain DLL on vital keratinocytes.

thumbnailFigure 1. Differentiation of keratinocytes results in a mixture of immature and semi mature cells. Keratinocytes of a representative donor were differentiated in chamber-slides for 6 days. Following fixation, cells were stained for the indicated cytokeratins (CK) using a panel of monoclonal (CK10, CK14, CK16) and polyclonal antibodies (pan). Duration of fluorescence detection was kept constant. Though variable, all cells were positive for CK14, while staining of CK10 and CK16 was limited to areas with blast-like keratinocytes.

thumbnailFigure 2. Analyses of DLL expression by Immunoblot and FACS using cell-lines over-expressing DLL. (A) Protein blots were prepared from TSt-4 thymic stromal cell-lines over-expressing human DLL1 or 4, and incubated with polyclonal antibodies specific for DLL. Both H20 and H265 only stained lanes containing TSt-4-DLL1 extracts, with a main band at 78 kDa. H265 stained additional bands at 50 kDa and 230 kDa, which could represent processed and glycosylated DLL-1, respectively. In contrast to H20, H265 also heavily stained the marker-lane, indicating that the specificity of this polyclonal antibody preparation is not limited to DLL. (B) Indicated cells were harvested using trypsin-EDTA, and incubated with H265 and H20 + control polyclonal antibodies specific for IRF1 and ACE. Control antibodies were selected based on species-origin and specificity for non-surface antigens. Both H265 and H20 showed weak, yet specific staining of TSt4-DLL1.

thumbnailFigure 3. 7AAD+ Keratinocytes show intense staining with any polyclonal primary antibody. Keratinocytes of donor C were harvested following high-density culture for 6 days, either in standard keratinocyte (iKer) or maturation medium (dKer). (A) All cells being stained with indicated rabbit pan antibodies were 7AAD+. Left column shows FACS plots as used by Clark et al. [15], right column contains additional information regarding viability. Within experiments, the percentage of positively stained cells was always similar with any pan-antibody. Insert shows background staining by the secondary antibody used. Even though staining intensity was lower after incubation with the secondary antibody, when compared with unstained cells (LL quadrant of the right column), the percentage of cells with signal was similar. This staining-pattern was observed for all donors. (B) When gated on 7AAD- cells, any pan antibody stained a low percentage of keratinocytes (donor C, donor D showed similar inconclusive staining profile). (C) Almost all cells that were stained with H265, co-stained with H20. A nearly similar plot could be obtained with any pan rabbit (eg ACE) and pan goat antibody combination (eg, NOS2), and invariably involved 7AAD+ cells (donor C, donor D showed similar co-staining).

Another question that arose was whether H265 is suitable for detection of intact DLL. Because the antibody was generated against a peptide spanning the membrane and the first twenty amino-acids of the extracellular domain, it may recognize processed DLL only (the epitope could be shielded by the 3D folding of the protein), thereby under-representing the actual level of DLL surface-expression. Therefore, using a goat-polyclonal antibody capable of binding to a known, accessible epitope in the extracellular domain of DLL1, H20, we repeated the experiments with DLL-transduced cells (Figure 2A and 2B) and keratinocytes (Figure 3C), but no specific staining on intact keratinocytes was detected. When considering the weak specific signals obtained with either H20 or H265 on cell-lines over-expressing DLL, it is obvious to conclude that it is impossible to demonstrate spontaneous DLL expression on intact primary cells with these antibodies.

Immunofluorescence

To confirm DLL expression by FACS, Clark et al. [16] used immunofluorescence on fixed keratinocytes prepared by cytospin, which is an application reported suitable for both H265 and H20. However, again no detection of any DLL staining of intact, differentiated keratinocytes was observed; the occasional green cell we did see (resembling Figure Five C in [16]) proved to have no nucleus (Figure 4A). In contrast, keratinocytes did stain positive for Notch ligand Jagged-1 (Figure 4A).

thumbnailFigure 4. Keratinocytes have very low expression of DLL. (A): Next to FACS, Clark et al. [15] used immuno-fluorescence to demonstrate DLL expression in immature keratinocyte-cultures. While the secondary antibody did not give any background staining (LL), there were occasional H265-FITC positive cells (UR), but these always proved to be DAPI negative. In contrast to DLL, immature keratinocytes were reliably stained for the Notch-ligand Jagged-1. (B) For PCR, 4 dilutions (5x-20x-80x-320x) were prepared from cDNA of mKer from 3 donors (A, B, and C), iKer from donor C. Positive controls were prepared from murine TSt-4 cells constitutively expressing DLL-1 or DLL-4. At 35 cycles, DLL4-specific PCR product was only detected in the 5x diluted samples, while mRNA encoding for Jagged-1 was easily amplified in 30 cycles.

PCR

Since it was not possible to reliably detect DLL1 or DLL4 protein, we reverted to PCR to find out whether matured keratinocytes are at least capable to express high level of DLL RNA. As shown in Figure 4B, compared with gene-products normally expressed by keratinocytes, cDNA encoding for DLL4 required 35 cycles before detection. Clearly, expression of DLL is far from abundant in differentiated or immature keratinocytes (Figure 4B). In contrast to DLL expression, the high level of Jagged-1 expression was confirmed by detecting PCR products after 30 cycles (Figure 4B). This observation is in line with a previous report demonstrating high levels of Jagged-1 expression at various stages of keratinocyte-development [18].

In summary, using three different methods we could not detect any DLL expression by keratinocytes, which makes it unlikely that HSC differentiation towards T lineage as observed by Clark et al. [16] is initiated by T-lineage commitment induced by DLL expressed on keratinocytes. Of course, we do not know whether (or why) keratinocytes start to express DLL during co-culture with fibroblasts, or whether DLL-expression by keratinocytes is actually required for the extra-thymic T cell development. On the other hand, keratinocytes do show abundant expression of Jagged-1. Even though Jagged-1 by itself is not particularly efficient in induction of T-lineage differentiation of cord blood derived CD34+ cells [19], and its expression in the murine thymus is not abundant [20,21], Jagged-1-mediated activation of murine thymic precursors does result in T/NK progenitors [20]. Therefore, it cannot be excluded that Jagged-1 does so as well in the context of the skin explants system.

A balanced co-culture?

The system requires co-culture of keratinocytes and fibroblasts on the Statamatrix®. However, "matured" keratinocytes showed limited capacity to adhere to collagen-coated Statamatrices®, in contrast to fibroblasts, and these latter cells always overgrew in keratinocyte-fibroblast co-cultures in matrices within 7 days (Figure 5A and 5B).

thumbnailFigure 5. Keratinocytes are out-competed by fibroblasts. (A) Matrix was stained for keratinocytes and fibroblasts using panCK and CD90, respectively, six days after matrices were seeded at a 2:1 ratio. At this stage, matrices were carefully seeded with HSC. (B) After 4 weeks, matrices were completely covered with CD90+ fibroblasts. HSC-derived cells could only be retrieved from the matrix following collagenase-dispase treatment. Fibroblasts from all donors grew similarly.

Skin explant procedure did not result in T lineage-development

We found that HSC (expressing either CD34+ or AC133+) were never able to differentiate towards T-lineage cells in matrices containing isolated and cultured fibroblasts and keratinocytes; most HSC did up-regulate CD7 and lost the CD34 marker, but no expression of early T-lineage markers like CD5 (Figure 6), nor the CD56 NK cell marker were observed (data not shown). In contrast, the same batches of HSC demonstrated excellent T lineage differentiation capacity when grown on murine stromal cell lines expressing human DLL [22].

thumbnailFigure 6. HSC expand, but do not differentiate in the skin-cell explants. HSC from donor V1 and V2 were seeded into skin-cell explants from donor A and B. As controls, HSC were expanded for 1 week using a HSC expansion-mix, followed by maintenance with IL-7. For reasons unknown, cell-numbers declined after 2-3 weeks in culture with skin-cell explants (not shown). The phenotype of the hematopoietic cells was analyzed at various time-points after seeding, and the results of week 2 and 4 are depicted. Of markers associated with T-lineage commitment, only CD7 was detected on 20-30%/60% of the cells in skin-cell explants/controls, and its expression-level increased from week 2 to 4.

DLL-independent development?

As indicated, the phenotype of the differentiated HSC suggested lack of Notch signaling, which could be due to the lowered keratinocyte/fibroblast ratio. Since it seems very difficult to alter that ratio with the published protocol, it may be difficult to routinely use the skin explants procedure. However, the explants system remains unique in the fact that it encompasses complete T cell development resulting in functional, single-positive CD4 and CD8 T cells, which includes the poorly understood process of positive/negative selection. Because canonical T-cell development roughly consists of 3 phases - 1) DLL-dependent T-lineage commitment, 2) DLL-independent beta-chain selection, and 3) DLL-(in)dependent positive/negative selection, we investigated whether T cell development could occur with cells that already have received DLL-signaling and are T/NK or completely T-lineage committed [22]. Unfortunately, when T/NK-lineage committed cells were seeded in skin-explants, most became NK-lineage committed CD56+ cells (Figure 7), in the presence of additional IL-15. Thymocyte-like cells were never detected. When IL-15 was omitted, the viability and yield of the cultures were reduced considerably, and most cells lost CD5 and iCD3 expression (Figure 7), indicating that even in the presence of IL-7 and Flt3-L, the keratinocyte/fibroblast co-cultures were not able to maintain the T-lineage committed status of cells. The few cells remaining also displayed a CD56+ phenotype.

thumbnailFigure 7. Skin-cell explants cannot maintain T-lineage committed cells. HSC from indicated donors were pre-differentiated on monolayers of thymic stromal cells expressing huDLL-1 (see figure 2). After 4 weeks, 55-65% of the cells were CD5+CD7+ (see insert UL), and > 90% iCD3+CD45RA+ (NS). 1 × 105 cells were seeded in skin-cell explants and cultured for 2 and 4 weeks. In the presence of IL15, the population expanded 10-15 ×. Already at 2 weeks, all cells had lost expression of CD5 and become CD56+ NK-lineage committed cells. The same phenotype was observed when IL-15 was omitted, although overall expansion was strongly reduced to 1-1,5 ×.

Discussion

Our results clearly demonstrate that the keratinocyte differentiation procedure results either in fragile cells that fail to adhere to any structure or plate, or cells that do adhere but are outcompeted by fibroblasts within a week. Furthermore, differentiated keratinocytes do not express detectable levels of DLL (protein nor RNA), and therefore the T-lineage commitment observed by Clark et al. [16] must be mediated by other factors. Jagged-1 may be responsible for steering development towards the T-lineage of a limited fraction of CD34+ cells in their co-culture system, because it is abundantly expressed by keratinocytes. As stated in the results section, certain T lineage committed stadia can complete their development into DP and SP T cells independent of Notch signaling [23].

However, even if T cells can be generated ex vivo using the skin explants system, the question remains whether they will be functional. Extra-thymic development of T cells has been described for Oncostatin M (OncM) transgenic mice, where LNs take over the function of the thymus [24]. Even though in OncM mice the T cells appear to go through the same developmental stages as regular thymocytes, they are less functional, which may be the result of positive selection by other hematopoietic cells instead of thymic epithelium [25]. This, however, remains to be elucidated for T cells derived in any in vitro cell differentiation system.

The past decade knowledge-driven research has revealed how T-lineage commitment can be induced in HSC in vitro, using cell-lines transfected with huDLL [8,26,27], or, to a certain extent, with recombinant huDLL [28,29]. In contrast to mouse, differentiation of human progenitors rarely get past the DP-stage of thymocyte development, indicating it is difficult to push them through the positive-negative selection process. Interestingly, experiments with human T-progenitors sorted as or arrested at a DN2-3-like stage, obtained from either OP9-DLL or TSt-4-DLL - HSC co-cultures, respectively, demonstrated that these cells were capable of rapidly reconstituting the thymus of Rag2-/-γc-/- mice, where they completed their maturation [22,30]. Similar experiments with murine T-progenitors have shown that this approach considerably enhanced the generation of functional T cells after HSC transplantation [31,32]. This approach will certainly be applicable to patients in the near future, but still relies on the functional capacity of the thymus, which is limited in older patients. In this regard, more pragmatic research has resulted in several viable approaches capable of improving thymic function [33-35], which are now being tested in clinical trials [36,37]. The solutions to both problems concerning T cell reconstitution are expected to meet within the next decade.

Conclusions

The results described by Clark et al. [15] are fascinating and unique, but remain elusive. The results in the current paper show that by following the published procedure we could not obtain enough evidence to support the basic skin cell co-culture conditions suggested to be required for HSC differentiation towards mature T cells. Recent advancements in the field of T cell lineage differentiation and thymic rejuvenation will generate alternative procedures to reconstitute the T cell population following HSC transplantation of man.

Methods

Cells

Abdominal or breast-skin was obtained from healthy individuals (donor A-E) undergoing reconstructive surgery, and processed within 24 hours. When required, skin was kept in RPMI 1640 (Sigma-Aldrich Co., St. Louis, MO, USA) supplemented with 100 IU/ml penicillin, 100 μg/ml streptomycin (P/S) and 0,5 μg/ml fungizone (all from Invitrogen Ltd., Paisley, UK) until processing. After removal of subcutaneous fat (if applicable), skin was cut into small fragments and incubated in phosphate buffered saline (PBS, Sigma) containing 2.4 U/mL Dispase II (Roche, Indianapolis, IL, USA) overnight at 4°C. Epidermis and dermis were separated by tweezers.

To isolate keratinocytes, up to 20 epidermal sheets were digested using 2 ml Trypsin/EDTA (Lonza, Verviers, Belgium) for 5 minutes at 37°C. Following quick neutralization by Trypsin Neutralizing solution (TNS, Lonza), suspension was vortexed after which undigested tissue was removed by subsequent filtration through 500 μm (Nedfilter, Almere, The Netherlands) and 70 μm strainers (BD Biosciences, Erembodegem, Belgium). Cells were spun down at 250 g for 10', and resuspended at approximately 2 × 105 cells/ml in Keratinocyte-SFM medium containing 1% P/S, 0.39 mM CaCl2, bovine pituitary extract (BPS) and epidermal growth factor (EGF) according to the manufacturer's descriptions (keratinocyte medium, Invitrogen). Medium was changed after 2-3 days, and islands appeared after 1-2 weeks. Keratinocytes were passed to new flasks when islands contained 30-50 cells; overall density of flasks was kept at 30-40%. For experiments, keratinocytes used were passaged 6x or less.

To isolate fibroblasts, 10-20 dermal fragments were incubated at 37°C for 1 hour in DMEM/F12 (Invitrogen) medium containing 2.5 mg/mL Trypsin, 0.2 U/mL Liberase Bz3, and 0.2 mg/ml DNAse. Following neutralization with DMEM/F12 containing 15% fetal calf serum (FCS, Greiner Bio-one, Solingen, Germany), cell-suspensions were filtrated and spun down as described above. Cells were seeded at 2 × 105 cells/ml in DMEM/F12 containing P/S, 15% FCS and 10 ng/ml EGF (fibroblast medium). Fibroblast cultures were split when density reached 100%. These cells were never split more than 8x before use in co-cultures.

Mobilized CD34+ stem cells

CD34+ cells were isolated from peripheral blood mononuclear cells (PBMC) obtained from healthy volunteers (V1-3) treated with G-CSF (Neupogen, Amgen Inc., Thousand Oaks, CA, USA) at MUMC+ (Maastricht, The Netherlands). Isolation was performed with the Isolex 300i Magnetic cell selection system v2.5 (Baxter oncology, Brussels, Belgium) using the Isolex stem cell reagent kit (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) according to the manufacturer's instructions. The positive fraction containing > 94% CD34+ cells was frozen at a concentration of 5 × 106 cells per ml per vial. CD133+ cell preparations were obtained from Lonza.

Skin and CD34+ stem cells were obtained according to protocols approved by, and guidelines stipulated by the local medical ethical committee, and with consent from the donors.

Maturation/differentiation of keratinocytes

Keratinocytes at 30-40% density were harvested using Trypsin/EDTA and TNS (Lonza), quantified and seeded at 100% density in a 1:1 (vol/vol) mix of Keratinocyte-SFM and DF-K medium. DF-K medium is a 1:1 (vol/vol) mix of DMEM and Ham's F12 containing P/S, 0.2 ng/ml EGF, 25 μg/ml BPS and 1.5 mM L-Glutamine (Invitrogen). Medium was refreshed daily. This procedure only worked for keratinocytes isolated from breast-skin and not for abdominal skin.

The skin cell construct

Sterilized Statamatrix® (Cytomatrix, Australia) were coated by incubation in PBS containing 100 μg/ml rat tail collagen I (Roche) at 37°C, after which the coated matrices were washed twice in PBS, and maintained in PBS until use. Prior to use, matrices were transferred to non-tissue culture treated petridishes (Greiner), and PBS was aspirated. Keratinocytes and fibroblasts were harvested, resuspended in a 1:1 mixture of keratinocyte and fibroblast-medium (ker-fib medium), and quantified. Subsequently, keratinocytes and fibroblasts were combined at 2 × 106 and 1 × 106 cells/ml, respectively, and 50-100 μl of this mix was dripped onto a matrix. After 3 hours at 37°C, 5% CO2, matrices were moved to 24-well plates and 2 ml ker-fib medium was added. The skin cell constructs were cultured for 6 days and medium was changed every other day.

Seeding of CD34+/CD133+ cells

After 6 days, medium was replaced by IMDM (Invitrogen) supplemented with 10% FCS (Greiner), 20 ng/ml IL-7 and IL-15, and 100 ng/ml Flt3-L (all R & D systems, Abingdon, UK), then 1 × 104 CD34+ or CD133+ cells were dripped onto each matrix. Halve medium change was done 3 times weekly.

TSt-4

Thymic stromal cell lines TSt-4 and TSt-4 transduced with hDLL1 or hDLL4 were kindly supplied by Dr. H. Kawamoto (RCAI-RIKEN, Yokohama, Japan) and maintained in RPMI 1640 containing 5% fetal bovine serum (FBS), 1% PS, 1 mM sodium pyruvate, 0.1 mM MEM non essential amino acids (NEAA), and 5 × 10-5 M 2-mercaptoethanol (bME)(all from Invitrogen Ltd., Paisley, UK).

Flowcytometry

All antibodies, materials and equipment were obtained from BD Biosciences unless stated otherwise, and were used according to the manufacturer's instructions. At different time points, cells were analyzed using different combinations of fluorescein isothiocyanate (FITC), R-phycoerithrin (PE), peridinin chlorophyll-a protein (PerCP)-, and allophycocyanin (APC) conjugated monoclonal antibodies (mAbs). The following antibodies (clones) were used: CD1a (HI149), CD3 (UCHT1, SK7), CD4 (SK3), CD5 (UCHT2, L17F12, MEM32 - Immunotools, Friesoythe, Germany), CD7 (M-T701, 7F3 - Sanquin), CD8a (HIT8a, RPA T8), CD14 (M5E2), CD19 (HIB19), CD34 (8G12), CD38 (HB7), CD45 (2D1, HI30), CD45RA (HI100), CD46 (E4.3), CD56 (B159), CD90 (AS02, Dianova, Hamburg, Germany), CD271 (C40-1457), IFN-γ (25723.11), NKG2A (131411, R&D Systems, Minneapolis, MN, USA), NKp46 (9E2), TCRαβ (IP26 - eBioscience), and TCRγδ (B1.1 - eBioscience). Unconjugated goat and rabbit antibodies used were DLL1 (#H20 and #H265), Nitric Oxide Synthase 2 (NOS2), IFN-γ Responsive Factor 1 (IRF1), Angiotensin-converting enzyme (ACE) (all Santa Cruz Biotechnology, Santa Cruz, CA, USA), for which the following conjugates were used according to the manufacturer's instructions: donkey anti-goat FITC and donkey anti-rabbit FITC or APC (all Jackson ImmunoResearch, West Grove, PA, USA). 7-amino-actinomycin D (7AAD) was used to differentiate between viable and dead cells. For intracellular stainings, cells were permeabilized using perm/wash. Cells were analyzed on a FACSCalibur or FACScan with WinMDI (Joe Trotter, http://facs.scripps.edu/ webcite) software.

Sorting

Prior to differentiation, CD34+ cell preparations were depleted of CD38bright cells and contaminating T and NK cells by cell-sorting using conjugated anti-CD3, -CD4, -CD8, -CD38 and -CD56 antibodies. All CD34+ cell preparations had ≤ 0.05% contaminating mature lymphoid cells. Sorting was performed on a FACSAria (BD Biosciences) with FACSDiva software.

Immunofluorescence

Upon aspiration of media, monolayers or cells were washed with PBS and fixed with cold methanol: acetone (Merck) 1:1 for 10 minutes on ice. Following fixation, preparations were washed 3 times with PBS at RT, with each wash for 4 minutes on an orbital shaker. Blocking was done with for 30' with 1% normal donkey serum (JIR) in PBS, after which preparations were washed twice with PBS. The following primary antibodies were used, each at the proper, predetermined dilution: CD46 (BD Biosciences), CD90 (Dianova), DLL1, Jagged-1 (both Santa Cruz), pan-cytokeratin (Acris, Hiddenhausen, Germany), Keratin-10 (RKSE60), -14 (RCK107) and -16 (LL025)(all MuBio, Maastricht, The Netherlands). Preparations were incubated with primary antibodies for 1 hour at RT in the dark, after which they were washed 5 times with PBS, followed by incubation with secondary antibody for 1 hr at RT in the dark. Appropriate Texas Red or FITC-labeled donkey anti-rabbit anti-goat, or anti-mouse antibodies were obtained from Jackson Immunoresearch. Following another washing procedure, preparations were post-fixed for 15' with 2% paraformaldehyde in PBS. Then, preparations were washed twice, and covered with mounting medium containing 4',6-diamidino-2-phenylindole (Vector Laboratories, Burlingame, CA, USA). Preparations were analyzed using an Axioplan 2 microscope and Axiovision software (Zeiss, Jena, Germany).

PCR analysis

RNA was extracted from immature and matured keratinocytes using Trizol according to the manufacturer's instructions (Invitrogen). Following quantification and DNAse treatment, cDNA was synthesized using Superscript III according to the manufacturer's instructions (Invitrogen). PCR was carried out in 20 μl reaction volumes containing ≤ 80 ng or 40 ng RNA equivalent from keratinocytes or TSt-4 cells, respectively. All PCR components were used according to the manufacturer's instructions (iTaq, BioRad, Hercules, CA, USA) 100 nM of each primer (Eurogentec, Liege, Belgium). Primers used were (anneal temperature, optimal MgCl2): Keratin-14 f-CACCTCTCCTCCTCCCAGTT r-CATCGTGCACATCCATGAC (63°C, 3 mM), DLL-1 f-CGTCGACTCCTTCAGTCTGC r-TTCTGTTGCGAGGTCATCAG (60.5°C, 3 mM), DLL-4 f-TCCAACTGCCCTTCAATTTC r-ACTGCAGATGACCCGGTAAG (57°C, 5 mM), and Jagged-1 f-CGGCCTCTGAAGAACAGAAC r-CCTCAGAGGCTGAGTGTGTG (62°C, 3 mM). All PCR products were validated by TA-cloning and sequencing according to standard procedures.

Abbreviations

CK: cytokeratins; DLL: Delta-like ligand; HSC: Hematopoietic stem cells

Authors' contributions

BM; designed and performed experiments, and designed and wrote the paper. CHMJvE; designed and performed experiments, and helped writing the paper. MJAJH, SJCvdS, ST, and SBJL; performed experiments. JV; monitored the project and analyzed the data. RH; organized research at PharmaCell. MK; designed the project. WTVG; designed the project, designed experiments and wrote the paper. GMJB; supervised the project. All authors read and approved the final manuscript.

A signed response to: T cells fail to develop in the human skin-cell explants system; an inconvenient truth

Rachael A. Clare and Thomas S. Kupper

Department of Dermatology, Brigham and Women's Hospital, 221 Longwood Avenue, Boston, MA, USA

Response

The authors describe studies attempting to generate human T cells in the cultured skin system we described previously [38]. Their efforts failed to result in a robust population of keratinocytes on the three-dimensional matrix and they failed to observe T cell differentiation. First and foremost, we agree that if there is not a viable robust population of keratinocytes, T cell differentiation will not occur. We have never observed successful T cell generation in this system in without viable and adherent keratinocytes that were at least as abundant as fibroblasts. We believe that the failure of keratinocytes to adhere and grow on the matrices in the authors' hands is primarily a technical issue. Working with keratinocytes can be difficult. These cells need to be passaged at a very low density, under which they maintain a basal cell-like phenotype. Once these cells reach a high enough density or are exposed to other differentiation signals, they can lose their ability adhere and proliferate. As a brief technical note, we have found it is best to plate cells onto the matrices while the keratinocytes are in a basal cell-like phenotype and then allow them to expand and differentiate in situ on the matrix. This produces the desired population of mature keratinocytes that are an absolute requirement for observing T cell differentiation in the system. These are technical aspects we would've been happy to share with the authors.

In the presence of viable keratinocytes, we do observe T cell generation in the system. We absolutely stand by our results, which are genuine. However, work over the last several years has made us less enthusiastic about this system for different reasons. First, the percentage of viable T cells was never higher than 10% of the total output of the matrices, even under the best conditions. This was not enough to for clinical treatment of patients. In order to scale the system up, we attempted to transition to a two-dimensional culture system using fibroblasts and keratinocytes. Under these conditions, the skin cells grew well but T cell precursors only matured to the double positive stage. Despite a great deal of effort, we were unable to generate single positive T cells in the two-dimensional system. Some aspect of the three-dimensional system appears to be required. We transduced human fibroblasts with human Delta-like ligand 1 (DLL-1) in the hopes of eliminating the need for human keratinocytes in this system, but this was also not successful in producing mature, single positive T cells. The Statamatrix three-dimensional environment has actually been shown to support the survival and multipotentcy of human hematopoietic precursor cells, although the mechanism for this remains unclear [39]. Although the need for three-dimensional environment is an interesting biologic question, we were focused on generating T cells that could be used in patients and so left this question for examination by our colleagues working on T cell differentiation as their primary research focus.

Subsequent to the publication of our manuscript, we also developed concerns about the specificity of the H265 antibody. We generated human fibroblasts transduced with human DLL-1 and found that cells expressing DLL-1 by RT-PCR were not reliably detected by flow cytometry with the H265 antibody, in agreement with findings reported here by the authors. We discontinued using this antibody as a result. At the time of our initial studies, this was the best DLL-1 antibody available. However, the inadequacy of this commercially available antibody does not change the fact that T cells develop in the system.

We share the authors' frustration that there is no good system to generate naïve autologous T cells for use in our patients after stem cell transplantation. In the presence of a viable and robust population of keratinocytes, the explant system does support T cell differentiation but we find that it supports the development of too few T cells to be useful clinically. After several years spent attempting to scale up the system for more significant T cell production, we chose to move on to more promising avenues of research. Once the signals that support human T cell differentiation are more clearly defined, it may be worth revisiting. However, we stand by our results, which were genuine and reproducible.

One thing the authors and others may find of interest are some recent findings in the field of peripheral tissue immunity. We and other have found that T cells generated during a cutaneous immune response home to the skin and remain there long term as a resident cell population [40-42]. The normal skin surface of an adult individual contains nearly 20 billion antigen-experienced memory T cells, each of which is the product of a previous infectious challenge [43]. Similar resident populations of T cells exist in the gut and the lung [44]. We would argue that these T cells, each of which is specific for a known pathogen encountered in the past, have the potential to be more valuable for restoring immunity in patients after transplantation. Even if we could generate millions of autologous naïve T cells, only a very small fraction of these would be specific for pathogens actually encountered by our patients. A better approach may be to transfer the established memory T cells from the hematopoetic stem cell donor to the recipient. We can expand 500,000 T cells from a 0.4cm2 skin biopsy [45]. These cells are antigen-experienced memory T cells with a substantial proliferative capacity and impressive effector functions [43]. We would argue that transferring these T cells, harvested and expanded from a small skin biopsy taken at the time of stem cell harvest, could much more meaningful way to address the immunosuppression our patients face after stem cell transplantation.

In short, we agree with the authors that in the absence of viable keratinocytes, there is no T cell development in the system. We believe that the failure of keratinocytes to populate the explant system in the authors hands is primarily a technical issue, one we would have been happy to help them address. However, even under the best conditions, the system only produces a small number of T cells. Several years of effort in scaling up the system or adapting it to two-dimensional system have failed, at least in our hands, to generate clinically significant numbers of T cells. Additional information on the process of T cell development in humans is needed before we can generate large number of naïve T cells in culture.

The authors and our team have the common goal of improving immunity in our patients after hematopoetic stem cell transplantation. It is unfortunate that the authors, instead of contacting us for assistance in getting keratinocytes to adhere and proliferate in their system, chose to write a manuscript describing the fact that when our system is inadequately reproduced, the results are not the same. We would argue that this is not surprising. However, the results we reported in our initial manuscript are absolutely genuine. If the authors wish, we would be happy to help them address these technical issues, although we believe that expansion and transfer of antigen experienced tissue resident T cells will prove to be a better approach in the long run to restoring patient immunity.

Acknowledgements

Prof. Dr. Frans Ramaekers (MuBio and University Maastricht, the Netherlands) kindly provided the RKSE60, RCK107 and LL025 anti-keratin antibodies. This work was supported by SenterNovem (Project: IS055002 to WTVG and GMJB).

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