Oral antigen-induced anaphylaxis in 129S5 and BALB/c mice is histamine-dependent. (a) Maximal rectal temperature change at 30 minutes following OVA gavage of OVA-sensitized 129S5 and BALB/c mice. (b) Maximal rectal temperature change at 30 minutes following OVA gavage of OVA-sensitized 129S5 and BALB/c mice treated with H1 and H2-receptor antagonists. Mice were sensitized with OVA/Alum (50 μg/2 mg) subcutaneously. Two weeks later, after 4 hours starvation, mice received oral gavage of OVA (50 mg/250 μl) saline and rectal temperatures were recorded at 30 and 60 minutes using rectal thermometry. b) Mice were i.p injected with anti-histamine (0.2 mg Triprolidine + 0.2 mg Cimetidine/200 μl saline) 30 minutes before prior to OVA- challenge and rectal temperatures determined at 30 min and 60 minutes following OVA gavage. (n = 8) Data represent mean ± SEM. Data were analyzed by one-way analysis of variance (ANOVA) and a post-hoc comparison test (Tukey-Kramer). Single and triple asterisks indicate a P value less than < 0.01 and < 0.001, respectively.
Arumugam et al. BMC Immunology 2011 12:14 doi:10.1186/1471-2172-12-14