Tumor growth and myeloid infiltrate in mice lacking IL-10 signaling. A. TC-1 cells, 105 cells/mouse were injected into IL-10 KO (gray triangles), wild type C57BL/6 mice (black diamonds), C57BL/6 mice treated (intraperitoneal injections) with 10 μg/ml anti-IL-10 or 10 μg/ml anti-IL-10R in alternate doses, except for the first dose, which was anti-IL-10 together with anti-IL-10R in the same day as TC-1 cells were injected (gray circles) (some mice were treated in the same frequency with 500 μg of anti-IL-10R, clone 1b13a, included in the last group). The averages of tumor measurements of 12 mice, each condition, for the group of KO and wild type mice and 6 mice, each condition, for the group of antibody treated mice are represented; differences in tumor growth between control group C57BL/6 and IL-10 deficient mice (‡) or mice treated with neutralizing antibodies (*) were highly significant, in both cases p-value < 0.01. B and C. Analysis of tumor infiltrate by flow cytometry. Tumors were collected 15 or 17 days post injection. Total cell suspensions were stained with antibodies against the indicated markers and analyzed in a FACSCalibur. At least 30000 events were acquired per sample; * indicates significant differences between groups.
Bolpetti et al. BMC Immunology 2010 11:27 doi:10.1186/1471-2172-11-27