Cell cycle analysis of TC-1 cells treated with IL-10. A total of 25000 TC-1 cells were treated for 4 days with 0, 3, 30 or 300 ng/ml IL-10. After this period, cells were harvested, fixed, stained and analyzed in a FACSCalibur. A total of 50000 cells were acquired per sample (note that after 4 days in culture, TC-1 cells have exponentially grown, resulting in a total of about 1 million cells in the end of the experiment). No significant differences were observed between the different conditions.
Bolpetti et al. BMC Immunology 2010 11:27 doi:10.1186/1471-2172-11-27