Characterization of TC-1 derived tumors. A. Tumor growth kinetics after injection of 105 TC-1 cells in the dorsal flank of C57BL/6 mice. Results are the average of tumor measures from 9 mice. B. Histology of TC-1 tumors. Tumor cryo-sections stained with hematoxylin/eosin exemplifying areas of leukocyte infiltrate, circulated by doted lines (100 × magnification). Arrows indicate some of the blood vessels. C. Immunohistochemistry characterization of TC-1 tumors. Tumor cryo-sections were incubated with antibodies against the indicated molecules. Antigen-antibody reaction was detected through peroxidase/DAB staining. Positive controls for each antibody are in the insets in each panel (200 × magnification).
Bolpetti et al. BMC Immunology 2010 11:27 doi:10.1186/1471-2172-11-27