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Open Access Highly Accessed Research article

Interleukin-10 production by tumor infiltrating macrophages plays a role in Human Papillomavirus 16 tumor growth

Aline Bolpetti1, João S Silva2, Luisa L Villa3 and Ana Paula Lepique3*

Author Affiliations

1 Fundação Antônio Prudente; Rua Prof. Antonio Prudente, 409, São Paulo, SP, 01509-010, Brazil

2 Department of Biochemistry and Immunology, School of Medicine of Ribeirão Preto, University of São Paulo; Avenida Bandeirantes, 3900, Campus da USP, Fazenda Monte Alegre, Ribeirão Preto, SP, 14049-900, Brazil

3 Ludwig Institute for Cancer Research; Rua João Julião, 245, 1o andar, São Paulo, SP, 01323-903, Brazil

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BMC Immunology 2010, 11:27  doi:10.1186/1471-2172-11-27

Published: 7 June 2010

Abstract

Background

Human Papillomavirus, HPV, is the main etiological factor for cervical cancer. Different studies show that in women infected with HPV there is a positive correlation between lesion grade and number of infiltrating macrophages, as well as with IL-10 higher expression. Using a HPV16 associated tumor model in mice, TC-1, our laboratory has demonstrated that tumor infiltrating macrophages are M2-like, induce T cell regulatory phenotype and play an important role in tumor growth. M2 macrophages secrete several cytokines, among them IL-10, which has been shown to play a role in T cell suppression by tumor macrophages in other tumor models. In this work, we sought to establish if IL-10 is part of the mechanism by which HPV tumor associated macrophages induce T cell regulatory phenotype, inhibiting anti-tumor activity and facilitating tumor growth.

Results

TC-1 tumor cells do not express or respond to IL-10, but recruit leukocytes which, within the tumor environment, produce this cytokine. Using IL-10 deficient mice or blocking IL-10 signaling with neutralizing antibodies, we observed a significant reduction in tumor growth, an increase in tumor infiltration by HPV16 E7 specific CD8 lymphocytes, including a population positive for Granzyme B and Perforin expression, and a decrease in the percentage of HPV specific regulatory T cells in the lymph nodes.

Conclusions

Our data shows that in the HPV16 TC-1 tumor mouse model, IL-10 produced by tumor macrophages induce regulatory phenotype on T cells, an immune escape mechanism that facilitates tumor growth. Our results point to a possible mechanism behind the epidemiologic data that correlates higher IL-10 expression with risk of cervical cancer development in HPV infected women.