Table 2 |
||||||||
|
Total protein and protein concentrations in individual fractions obtained after differential and density centrifugation. |
||||||||
|
fraction |
Exp. 1 |
Exp. 2 |
Exp. 3 |
Exp. 4 |
mean |
SD |
Volume |
protein |
|
[mg/ml] |
[mg/ml] |
[mg/ml] |
[mg/ml] |
[mg/ml] |
[μl] |
[μg] |
||
|
|
||||||||
|
homogenate |
3.891 |
3.222 |
3.532 |
3.684 |
3.582 |
0.282 |
2700 |
9671.4 |
|
enriched organelles (input) |
0.745 |
0.651 |
0.729 |
0.865 |
0.747 |
0.088 |
1800 |
1345.3 |
|
fraction 1 |
0.349 |
0.303 |
0.347 |
0.296 |
0.324 |
0.028 |
200 |
64.7 |
|
fraction 2 |
0.430 |
0.468 |
0.306 |
0.389 |
0.398 |
0.069 |
400 |
159.3 |
|
fraction 3 |
0.764 |
0.909 |
1.004 |
0.879 |
0.889 |
0.099 |
300 |
266.7 |
|
fraction 4 |
0.526 |
0.548 |
0.480 |
0.405 |
0.490 |
0.063 |
300 |
147.0 |
|
fraction 5 |
0.925 |
0.882 |
0.959 |
0.852 |
0.905 |
0.047 |
200 |
180.9 |
|
fraction 6 |
0.384 |
0.431 |
0.352 |
0.349 |
0.379 |
0.038 |
200 |
75.8 |
|
|
||||||||
|
recovery |
894.4 |
|||||||
|
|
||||||||
|
Schmidt et al. BMC Immunology 2009 10:41 doi:10.1186/1471-2172-10-41 |
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