Purification of enriched secretory lysosomes. A. Enriched organelle fractions (EO) were adjusted to 19% (v/v) OptiPrep® and loaded in the middle of the discontinuous density gradient. B. After 5 h of ultracentrifugation, six individual fractions became apparent at the individual interphases that were collected, pelleted and used for subsequent analyses.
Schmidt et al. BMC Immunology 2009 10:41 doi:10.1186/1471-2172-10-41