Generation of SLY1 ko mice. A. Scheme of the sly1 genomic locus, targeting vector and inserted neomycin cassette after homologous recombination. The neomycin cassette is inserted in inverted orientation directly after the starting ATG. B. Screening PCR with genomic tail DNA and primers as described in material and methods. C. Southern blot after digestion of genomic tail DNA with EcoRV and detection with 32P-labeled probe depicted in A. D. Western Blot assessing the successful inactivation of the sly1 gene using two different SLY1-specific antibodies and β-actin as loading control.
Reis et al. BMC Immunology 2009 10:38 doi:10.1186/1471-2172-10-38