Promoter-GFP reporter genes confirm neural expression of transcripts from pan-neural enriched data sets. Transgenic animals expressing GFP reporters for representative transcripts exclusively enriched in either the IVT derived data set (A-D) or the WT-Pico-amplified sample (E-L). A. F32B6.11::GFP is expressed throughout the C. elegans nervous system including neurons associated with the Nerve ring in the head, motor neurons throughout the Ventral Nerve Cord (VNC) and in tail ganglia. D. F49H12.4::GFP is selectively expressed in PVD nociceptive neuron and in two additional neurons in the tail region. Note the highly branched PVD dendritic architecture (arrowheads). E. Differential Interference Contrast (DIC) image of midbody region of 2nd stage larva (F.) expressing F47B8.3::GFP in GABAergic motor neurons (DD5, VD10, VD11) in the ventral nerve cord. P9 and P10 denote landmark hypodermal blast cells. G-H. ZC155.2::GFP and C50F4.4::GFP are expressed in VNC motor neurons (e.g. VA10, VB11, etc). Anterior to left, Ventral down. VNC (Ventral Nerve Cord).
Watson et al. BMC Genomics 2008 9:84 doi:10.1186/1471-2164-9-84