A comprehensive collection of experimentally validated primers for Polymerase Chain Reaction quantitation of murine transcript abundance

doi:10.1186/1471-2164-9-633

BMC Genomics

Volume 9

Viewing options:

Abstract
Full text
PDF (474KB)
Additional files

Associated material:

Related literature:

Articles citing this article
on Google Scholar
on ISI Web of Science
on PubMed Central
Other articles by authors
on Google Scholar

Spandidos A
Wang X
Wang H
Dragnev S
Thurber T
Seed B

on PubMed

Spandidos A
Wang X
Wang H
Dragnev S
Thurber T
Seed B
Related articles/pages
on Google

on Google Scholar

on PubMed

Tools:

Post to:

Research article

A comprehensive collection of experimentally validated primers for Polymerase Chain Reaction quantitation of murine transcript abundance

Athanasia Spandidos¹^,2 , Xiaowei Wang¹^,2^,3 , Huajun Wang¹^,2 , Stefan Dragnev¹^,2^,4 , Tara Thurber¹^,2 and Brian Seed¹^,2

¹Center for Computational and Integrative Biology, Massachusetts General Hospital. MA, USA

²Department of Genetics, Harvard Medical School, 185 Cambridge Street, Boston, MA 02114-2790, USA

³Division of Bioinformatics and Outcomes Research, Department of Radiation Oncology, Washington University School of Medicine, 4921 Parkview Place, St. Louis, MO 63110, USA

⁴Idearc Media Corp, 1601 Trapelo Road, Waltham, MA 02451, USA

author email corresponding author email

BMC Genomics 2008, 9:633doi:10.1186/1471-2164-9-633


Published:	24 December 2008

Additional files

Additional file 1:

Five representative examples of primer pairs that were successful throughout the validation procedure.

Format: PDF Size: 798KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional file 2:

Five representative examples of primer pairs that failed based on agarose gel analysis.

Format: PDF Size: 854KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional file 3:

Five representative examples of primer pairs that failed based on BLAST analysis.

Format: PDF Size: 787KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional file 4:

Information for mouse primer pairs from PrimerBank tested using QPCR.

Format: PDF Size: 9KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional file 5:

NCBI BLAST analysis of successfully sequenced PCR products.

Format: PDF Size: 8KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional file 6:

Validation of 96 PrimerBank primer pairs which had failed QPCR during the high-throughput validation procedure.

Format: PDF Size: 800KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional file 7:

Validation of 96 PrimerBank primer pairs which had failed QPCR during the high-throughput validation procedure.

Format: PDF Size: 791KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional file 8:

Analysis of technical replicate experiments.

Format: PDF Size: 7KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional file 9:

Analysis of individual primer pairs from technical replicate experiments.

Format: PDF Size: 18KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional file 10:

Frequency distributions of log normal data from five technical replicate tests.

Format: PDF Size: 347KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional file 11:

Comparison of pipetting variation between manual and robotic liquid transfer.

Format: PDF Size: 7KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional file 12:

Amplicons used for SYBR Green I sequence specificity experiments.

Format: PDF Size: 7KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional file 13:

SYBR Green I binding to dsDNA of increasing length and AT%.

Format: PDF Size: 202KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional file 14:

Amplification efficiency estimation from single reaction kinetics data.

Format: PDF Size: 7KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional file 15:

Amplification efficiency estimation using analytical and standard curve methods.

Format: PDF Size: 10KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional file 16:

One-way ANOVA test to determine if amplification efficiency varies significantly between different PrimerBank primer pairs.

Format: PDF Size: 206KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional file 17:

PrimerBank primer pair groups used for one-way ANOVA analysis.

Format: PDF Size: 7KB Download file

This file can be viewed with: Adobe Acrobat Reader