Research article
A comprehensive collection of experimentally validated primers for Polymerase Chain Reaction quantitation of murine transcript abundance
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* Corresponding author: Brian Seed bseed@ccib.mgh.harvard.edu
1 Center for Computational and Integrative Biology, Massachusetts General Hospital. MA, USA
2 Department of Genetics, Harvard Medical School, 185 Cambridge Street, Boston, MA 02114-2790, USA
3 Division of Bioinformatics and Outcomes Research, Department of Radiation Oncology, Washington University School of Medicine, 4921 Parkview Place, St. Louis, MO 63110, USA
4 Idearc Media Corp, 1601 Trapelo Road, Waltham, MA 02451, USA
BMC Genomics 2008, 9:633 doi:10.1186/1471-2164-9-633
Published: 24 December 2008Additional files
Additional file 1:
Five representative examples of primer pairs that were successful throughout the validation procedure.
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Additional file 2:
Five representative examples of primer pairs that failed based on agarose gel analysis.
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Additional file 3:
Five representative examples of primer pairs that failed based on BLAST analysis.
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Additional file 4:
Information for mouse primer pairs from PrimerBank tested using QPCR.
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Additional file 5:
NCBI BLAST analysis of successfully sequenced PCR products.
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Additional file 6:
Validation of 96 PrimerBank primer pairs which had failed QPCR during the high-throughput validation procedure.
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Additional file 7:
Validation of 96 PrimerBank primer pairs which had failed QPCR during the high-throughput validation procedure.
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Additional file 8:
Analysis of technical replicate experiments.
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Additional file 9:
Analysis of individual primer pairs from technical replicate experiments.
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Additional file 10:
Frequency distributions of log normal data from five technical replicate tests.
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Additional file 11:
Comparison of pipetting variation between manual and robotic liquid transfer.
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Additional file 12:
Amplicons used for SYBR Green I sequence specificity experiments.
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Additional file 13:
SYBR Green I binding to dsDNA of increasing length and AT%.
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Additional file 14:
Amplification efficiency estimation from single reaction kinetics data.
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Additional file 15:
Amplification efficiency estimation using analytical and standard curve methods.
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Additional file 16:
One-way ANOVA test to determine if amplification efficiency varies significantly between different PrimerBank primer pairs.
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Additional file 17:
PrimerBank primer pair groups used for one-way ANOVA analysis.
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