Expression analysis of putative MFS genes by RT-PCR. The expression of 8 putative MFS genes, which were not validated by the mRNA/protein profiling data mining, was checked by RT-PCR. Purified poly(A)+ enriched mRNA fractionated from C. albicans isolate SC5314 were amplified by RT-PCR, as described in the Methods. Following electrophoresis through 1.2% agarose gel, the amplified PCR products were visualized by staining with ethidium bromide. Lane M, nucleotide size marker (PCR Marker); lane 1, without RT (negative control); lane 2, MDR1 (positive control, 330 base pairs); lane 3, orf19.1582; lane 4, orf19.7336; lane 5, orf19.4090; lane 6, orf19.6180; lane 7, orf19.1424; lane 8, orf19.6520; lane 9, orf19.6654 and lane 10, orf19.6976.
Gaur et al. BMC Genomics 2008 9:579 doi:10.1186/1471-2164-9-579