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Open AccessHighly AccessResearch article

Analysis of 4,664 high-quality sequence-finished poplar full-length cDNA clones and their utility for the discovery of genes responding to insect feeding

Steven G Ralph1,6* email, Hye Jung E Chun2* email, Dawn Cooper1 email, Robert Kirkpatrick2 email, Natalia Kolosova1,3 email, Lee Gunter4 email, Gerald A Tuskan4 email, Carl J Douglas3 email, Robert A Holt2 email, Steven JM Jones2 email, Marco A Marra2 email and Jörg Bohlmann1,3,5 email

Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, V6T 1Z4, Canada

British Columbia Cancer Agency Genome Sciences Centre, Vancouver, British Columbia, V5Z 4E6, Canada

Department of Botany, University of British Columbia, Vancouver, British Columbia, V6T 1Z4, Canada

Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, 37831, USA

Department of Forest Sciences, University of British Columbia, Vancouver, British Columbia, V6T 1Z4, Canada

Department of Biology, University of North Dakota, Grand Forks, North Dakota, 58202-9019, USA

author email corresponding author email* Contributed equally

BMC Genomics 2008, 9:57doi:10.1186/1471-2164-9-57

Published: 29 January 2008

Additional files

Additional file 1:

Full-length cDNA inventory. Predicted protein-coding features and annotation for the poplar full-length cDNA collection.

Format: XLS Size: 3.7MB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional file 2:

Microarray dataset. Poplar FLcDNAs mapped to the genome-wide transcript profile of poplar leaves 24 h after the onset of forest tent caterpillar feeding using a 15.5 K array.

Format: XLS Size: 4.5MB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional file 3:

Supplemental methods. Poplar methods for RNA isolation, full-length cDNA library construction, bacterial transformation with plasmids, clone handling, DNA purification and evaluation, and DNA sequencing are provided.

Format: DOC Size: 59KB Download file

This file can be viewed with: Microsoft Word Viewer


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