BMC Genomics

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Open Access Highly Access Research article

Analysis of 4,664 high-quality sequence-finished poplar full-length cDNA clones and their utility for the discovery of genes responding to insect feeding

Steven G Ralph1,6, Hye Jung E Chun2, Dawn Cooper1, Robert Kirkpatrick2, Natalia Kolosova1,3, Lee Gunter4, Gerald A Tuskan4, Carl J Douglas3, Robert A Holt2, Steven JM Jones2, Marco A Marra2 and Jörg Bohlmann1,3,5*

Author Affiliations

1 Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, V6T 1Z4, Canada

2 British Columbia Cancer Agency Genome Sciences Centre, Vancouver, British Columbia, V5Z 4E6, Canada

3 Department of Botany, University of British Columbia, Vancouver, British Columbia, V6T 1Z4, Canada

4 Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, 37831, USA

5 Department of Forest Sciences, University of British Columbia, Vancouver, British Columbia, V6T 1Z4, Canada

6 Department of Biology, University of North Dakota, Grand Forks, North Dakota, 58202-9019, USA

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BMC Genomics 2008, 9:57 doi:10.1186/1471-2164-9-57

Published: 29 January 2008

Additional files

Additional file 1:

Full-length cDNA inventory. Predicted protein-coding features and annotation for the poplar full-length cDNA collection.

Format: XLS Size: 3.7MB Download file

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Additional file 2:

Microarray dataset. Poplar FLcDNAs mapped to the genome-wide transcript profile of poplar leaves 24 h after the onset of forest tent caterpillar feeding using a 15.5 K array.

Format: XLS Size: 4.5MB Download file

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Open Data

Additional file 3:

Supplemental methods. Poplar methods for RNA isolation, full-length cDNA library construction, bacterial transformation with plasmids, clone handling, DNA purification and evaluation, and DNA sequencing are provided.

Format: DOC Size: 59KB Download file

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Open Data