Table 4

Primers used for MLST

Locus

Function

Forward primer

Reverse primer

Location (a)


dinB

DNA polymerase

5'-GTT TTC CCA GTC ACG ACG TTG TAT GAG AGG TGA GCA ATG CGT A-3'

5'-TTG TGA GCG GAT AAC AAT TTC CGT AGC CCC ATC GCT TCC AG-3'

282,284 – 282,734

icdA

Isocitrate dehydrogenase

5'-GTT TTC CCA GTC ACG ACG TTG TAA TTC GCT TCC CGG AAC ATT G-3'

5'-TTG TGA GCG GAT AAC AAT TTC ATG ATC GCG TCA CCA AAY TC-3'

1,118,658 – 1,187,173

pabB

p-aminobenzoate synthase

5'-GTT TTC CCA GTC ACG ACG TTG TAA ATC CAA TAT GAC CCG CGA G-3'

5'-TTG TGA GCG GAT AAC AAT TTC GGT TCC AGT TCG TCG ATA AT-3'

1,807,273 – 1,807,740

polB

Polymerase PolII

5'-GTT TTC CCA GTC ACG ACG TTG TAG GCG GCT ATG TGA TGG ATT C-3'

5'-TTG TGA GCG GAT AAC AAT TTC GGT TGG CAT CAG AAA ACG GC-3'

65,220 – 64, 773

putP

Proline permease

5'-GTT TTC CCA GTC ACG ACG TTG TAC TGT TTA ACC CGT GGA TTG C-3'

5'-TTG TGA GCG GAT AAC AAT TTC GCA TCG GCC TCG GCA AAG CG-3'

1,074,708 – 1,075,163

trpA

Tryptophan synthase subunit A

5'-GTT TTC CCA GTC ACG ACG TTG TAG CTA CGA ATC TCT GTT TGC C-3'

5'-TTG TGA GCG GAT AAC AAT TTC GCT TTC ATC GGT TGT ACA AA-3'

1,338,658 – 1,338,098

trpB

Tryptophan synthase subunit B

5'-GTT TTC CCA GTC ACG ACG TTG TAC ACT ATA TGC TGG GCA CCG C-3'

5'-TTG TGA GCG GAT AAC AAT TTC CCT CGT GCT TTC AAA ATA TC-3'

1,339,430 – 1,338,837

uidA

Beta-glucuronidase

5'-GTT TTC CCA GTC ACG ACG TTG TAC ATT ACG GCA AAG TGT GGG TCA AT-3'

5'-TTG TGA GCG GAT AAC AAT TTC CCA TCA GCA CGT TAT CGA ATC CTT-3'

1,615,010 – 1,614,411

OF

Sequencing primer (b)

5'-GTT TTC CCA GTC ACG ACG TTG T-3'

OR

Sequencing primer (b)

5'-TTG TGA GCG GAT AAC AAT TTC-3'


(a) Based on Escherichia coli strain 536, complete genome, GenBank accession CP000247.

(b) PCR primers have the corresponding universal sequence in 5'.

Jaureguy et al. BMC Genomics 2008 9:560   doi:10.1186/1471-2164-9-560

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