Table 2

Summary of salmonid ESTs and contig assemblies.



Atlantic
salmon
Rainbow
trout
Chinook
salmon
Sockeye
salmon
Brook
trout
Lake
whitefish
Grayling

Northern
pike
Rainbow
Smelt

# EST sequencesa
436629
246704
14535
12056
10051
10842
10975
3624
36785










Assembly Stage1b









     # contigs (2+)c
70,845
42423
2890
2480
4178
4464
4616
1074
9044
     # singletonsd
47,139
26935
6295
4118
1247
2510
1314
1346
7019
     # transcriptse
117,984
69358
9185
6598
5425
6974
5930
2420
16063










Assembly Stage2f









     # transcriptsg
81398
51199
8517
6200
4946
6446
5408
2380
12159
     # hitsh
29844
19266
3684
3561
1838
2314
1780
198
6139
     % with hitsi
37
38
43
57
37
36
33
8
50

a number of EST sequences for all of the species including those in GenBank

b Assembly stage 1 refers to PHRAP assembly using parameters 0.99 repeat_frequency and 100 minscore

c number of contigs with 2 or more sequences

d number of contigs with 1 sequence

e total number of transcripts including singletons

f Assembly stage 2 refers to PHRAP assembly using parameters 0.96 repeat_frequency and 300 minscore

g the number of transcripts that result from a re-assembly of all stage 1 transcripts using PHRAP parameters 96 repeat_frequency and 300 minscore

h number of transcripts that have a BLASTX hit of < 1e-10 to SwissProt/CDD databases.

i percent of stage 2 assembled transcripts that have a BLASTX hit.

Koop et al. BMC Genomics 2008 9:545   doi:10.1186/1471-2164-9-545