Table 1

Sequence and assembly statistics

Pyrosequencing ESTs

Sanger ESTs

Pyrosequencing + Sanger ESTs

Pyrosequencing assembly

Sanger assembly

Hybrid Assembly


No. sequences

90,664

9,578

90,664 + 9,578 = 100,242

35,507 (11,155 assemblies)

4,689 (1,584 assemblies)

34,495 (10,811 assemblies)

Total bases (bp)

17,315,041

4,811,851

22,126,892

8,274,073

2,796,219

9,552,701

Minimum length (bp)

100

100

100

100

100

100

Average length (bp)

190

502

220

233

596

276

Maximum length (bp)

425

1,002

1,002

1,385

4,283

4,471

%GC

50.3

51.1

50.5

51.3

53.3

51.7

%N

0.04

0

0.03

0.05

0

0.04


Cheung et al. BMC Genomics 2008 9:542   doi:10.1186/1471-2164-9-542

Open Data