Short-term genome evolution of Listeria monocytogenes in a non-controlled environment

doi:10.1186/1471-2164-9-539

BMC Genomics

Volume 9

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Orsi RH
Borowsky ML
Lauer P
Young SK
Nusbaum C
Galagan JE
Birren BW
Ivy RA
Sun Q
Graves LM
Swaminathan B
Wiedmann M

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Orsi RH
Borowsky ML
Lauer P
Young SK
Nusbaum C
Galagan JE
Birren BW
Ivy RA
Sun Q
Graves LM
Swaminathan B
Wiedmann M
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Research article

Short-term genome evolution of Listeria monocytogenes in a non-controlled environment

Renato H Orsi¹ , Mark L Borowsky²^,7 , Peter Lauer³ , Sarah K Young² , Chad Nusbaum² , James E Galagan²^,4 , Bruce W Birren² , Reid A Ivy¹ , Qi Sun⁵ , Lewis M Graves⁶ , Bala Swaminathan⁶ and Martin Wiedmann¹

¹Department of Food Science, Cornell University, Ithaca, USA

²Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, Cambridge, USA

³Anza Therapeutics, Concord, USA

⁴Department of Biomedical Engineering and Microbiology, Boston University, USA

⁵Computational Biology Services Unit, Center for Advanced Computing, Cornell University, Ithaca, USA

⁶Enteric Diseases Laboratory Branch, Division of Foodborne, Bacterial and Mycotic Diseases, Centers for Disease Control and Prevention, Atlanta, USA

⁷Department of Molecular Biology, Massachusetts General Hospital, Boston, USA

author email corresponding author email

BMC Genomics 2008, 9:539doi:10.1186/1471-2164-9-539


Published:	13 November 2008

Additional files

Additional file 1:

Intracellular growth of isolates J2818 and J0161 in activated J774 macrophage cells. Graph of intracellular growth in activated macrophages of isolates J2818 and J0161.

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Additional file 2:

Comparison of comK prophage genes present in the human clinical and food isolates associated with the 1988 sporadic listeriosis case and the 2000 listeriosis outbreak. This table provides a gene-by-gene analysis of the comK prophage in strain F6854 (representing the 1988 isolates), J2818 (representing the 2000 isolates), and other L. monocytogenes strains.

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Additional file 3:

Primers used for validation of polymorphisms. List of primers used for validation of polymorphisms identified during genome analysis.

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Additional file 4:

Validation of the 43 polymorphic sites in the backbone alignment and the single polymorphic site in the tRNA prophage that were initially identified in the genome comparisons. This table describes the 44 polymorphisms identified in the genome analysis, including whether a given polymorphism was confirmed by PCR amplification and subsequent sequencing of the PCR product.

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