Research article
Short-term genome evolution of Listeria monocytogenes in a non-controlled environment
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* Corresponding author: Martin Wiedmann mw16@cornell.edu
1 Department of Food Science, Cornell University, Ithaca, USA
2 Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, Cambridge, USA
3 Anza Therapeutics, Concord, USA
4 Department of Biomedical Engineering and Microbiology, Boston University, USA
5 Computational Biology Services Unit, Center for Advanced Computing, Cornell University, Ithaca, USA
6 Enteric Diseases Laboratory Branch, Division of Foodborne, Bacterial and Mycotic Diseases, Centers for Disease Control and Prevention, Atlanta, USA
7 Department of Molecular Biology, Massachusetts General Hospital, Boston, USA
BMC Genomics 2008, 9:539 doi:10.1186/1471-2164-9-539
Published: 13 November 2008Additional files
Additional file 1:
Intracellular growth of isolates J2818 and J0161 in activated J774 macrophage cells. Graph of intracellular growth in activated macrophages of isolates J2818 and J0161.
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Additional file 2:
Comparison of comK prophage genes present in the human clinical and food isolates associated with the 1988 sporadic listeriosis case and the 2000 listeriosis outbreak. This table provides a gene-by-gene analysis of the comK prophage in strain F6854 (representing the 1988 isolates), J2818 (representing the 2000 isolates), and other L. monocytogenes strains.
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Additional file 3:
Primers used for validation of polymorphisms. List of primers used for validation of polymorphisms identified during genome analysis.
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Additional file 4:
Validation of the 43 polymorphic sites in the backbone alignment and the single polymorphic site in the tRNA prophage that were initially identified in the genome comparisons. This table describes the 44 polymorphisms identified in the genome analysis, including whether a given polymorphism was confirmed by PCR amplification and subsequent sequencing of the PCR product.
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