Figure 4.

Comparison of nucleosome positioning signals of histone methylation ChIP-Seq data and nucleosome sequencing data in functionally important regions. Examples comparing nucleosome positioning signals from ChIP-Seq and nucleosome sequencing at regions around CTCF binding site (A), DNase I HS site (B) and TSS (C). The nucleosome positioning signal of methylation ChIP-Seq (combining 21 types of histone modifications) is shown in red color; while that of nucleosome sequencing is shown in blue color. Each tag is extended towards 3' to 150 nt and taken the middle 75 nt to generate the positioning signals; wavelet denoising was used to smooth the signals. The black profiles under the nucleosome positioning signals of methylation ChIP-Seq and nucleosome sequencing indicate a CTCF binding site (A), DNase I HS site (B), and the promoter of C2CD2 (C), respectively.