Table 3

Molecular Analysis of Two Adjacent Helitrons

Inbred Line

F1 + R1

F3 + R3

F2 + R4

F4 + R4

Conclusions

4Co63

x

x

N/A

1 kb

A188

x

x

0.7 kb

N/A

No.1^-No.2^-

A636

3 kb

0.6 kb

x

x

No.1⁺No.2⁺

B73

3 kb

0.6 kb

x

1 kb

No.1⁺No.2⁺

BSSS53

x

x

N/A

N/A

McC

0.5 kb

0.6 kb

x

0.7 kb

No.1^-No.2⁺

CML139

x

x

0.7 kb

N/A

No.1^-No.2^-

H99

x

x

0.7 kb

x

No.1^-No.2^-

I137TN

x

N/A

N/A

1 kb

Ki3

x

x

0.7 kb

N/A

No.1^-No.2^-

M14

x

x

0.7 kb

N/A

No.1^-No.2^-

Mo17

x

x

0.7 kb

N/A

No.1^-No.2^-

W22

0.5 kb

0.6 kb

x

N/A

No.1^-No.2⁺

W23

0.5 kb

0.6 kb

N/A

N/A

No.1^-No.2⁺

PCR results from different primer combinations.

⁺: Helitron present

^-: Helitron absent

x: no PCR amplification

N/A: no PCR test

Conclusions were based on PCR results. Both No.1 and No.2 Helitrons were detected in lines A636 and B73, only Helitron No.2 in lines McC, W22, and W23, and neither Helitron in lines A188, CML139, H99, Ki3, M14, or Mo17. No conclusion could be reached for 4Co63, BSSS53, and I137 TN based on the above PCR results.

Du et al. BMC Genomics 2008 9:51   doi:10.1186/1471-2164-9-51