Table 5

Primers used for Sybr green qPCR

F9 cells

Gene

Forward

Reverse

GAPDH

5'-GCCAAGAGGGTCATCATCTCC-3'

5'-TTGGTTCACACCCATCACAAA-3'

MVD

5'-AGCATCGCCCGGCAG-3'

5'-TGGCCCCTGTAATTTCCCA-3'

LSS

5'-GCGGCTGTGCGATGCT-3'

5'-AGGTAGCGAACCCGCCA-3'

TGFB1

5'-TGGAAAGGGCCCAGCAC-3'

5'-GCAATAGTTGGTATCCAGGGCT-3'

IGFII

5'-AAGAGTTCAGAGAGGCCAAACG-3'

5'-ATCTCCGAAGAGGCTCCCC-3'

MT1

5'-TGCTCCACCGGCGG-3'

5'-TTTGCAGACACAGCCCTGG-3'

WNT6

5'-GGGCGCTGTCTGAGTCCA-3'

5'-TGGCCCCTGTAATTTCCCA-3'

tPA

5'-GGCCTGGCACGACACAAT-3'

5'-CATCACATGGCACCAAGGTC-3'

VEGFC

5'-CAGCTGCGGAAAGGCG-3'

5'-TTTACACTGTCCCCTGTCCTGG-3'

DHODH

5'-AACACAGGCTACGGGCCAG-3'

5'-TCCCAGAGGCAGGCCCAT-3'

HepG2 cells

Gene

Forward

Reverse

ACADM

5'-AGCTACCAAGTATGCCCTGGAA-3'

5'-TAAATGATATTGCTTGGTGCTCTACA-3'

ACAT2

5'-TGGGCCACCCTCTTGGA-3'

5'-CCAGTGTGTGTAACAGGGTCACA-3'

ACACA

5'-GCTCCTTGTCACCTGCTTCTG-3'

5'-TGTAGGCTAGAGATCCCCAAATCA-3'

APOA5

5'-AGGTGCGCCAGCGACTT-3'

5'-GCGAGTGAAGGCAGCTATCTG-3'

APOC1

5'-CAAGGCTCGGGAACTCATCA-3'

5'-CCCGCATCTTGGCAGAAA-3'

APOE

5'-CGCTGGGTGCAGACACTGT-3'

5'-AGGCCTTCAACTCCTTCATGGT-3'

APOL1

5'-TCAGCTGAAAGCGGTGAACA-3'

5'-CTCTGCTCATTTCCAGGATGCT-3'

CYP27A1

5'-CCCTGTGGTCCCCACAAA-3'

5'-GGAAGCCATCAACTTCAATTTCC-3'

HMGCR

5'-CCTGTAACTCAGAGGGTCAAGATGAT-3'

5'-CCAGCGACTGTGAGCATGAA-3'

HMGCS

5'-TCTTAAATCAAGGCTTGATTCAAGAA-3'

5'-TGTCCTCTCTGAGCTTCATGTTTT-3'

SREBF2

5'-CGAATTGAAAGACCTGGTCATG-3'

5'-TCCTCAGAACGCCAGACTTGT-3'

FABP

5'-CCGCTGGGTCCAAAGTGAT-3'

5'-CATTGTCTCCAGCTCACATTCC-3'

FASN

5'-GCAAATTCGACCTTTCTCAGAAC-3'

5'-GGACCCCGTGGAATGTCA-3'

LDLR

5'-AGATAGTGACAATGTCTCACCAAGCT-3'

5'-CTCACGCTACTGGGCTTCTTCT-3'

PPARG

5'-GCGAAAGCCTTTTGGTGACT-3'

5'-CAGTGCATTGAACTTCACAGCAA-3'

TYMS

5'-AATCACATCGAGCCACTGAAAA-3'

5'-AATCCTGAGCTTTGGGAAAGGGT-3'

Chittur et al. BMC Genomics 2008 9:507   doi:10.1186/1471-2164-9-507