Table 1

Generalised linear models for a) amplification success and b) polymorphism of conserved microsatellite loci

a) Amplification success1

b) Polymorphism2

Error

Residual

B

t

P

Error

Residual

B

t

P

df *

deviance*

df *

deviance*


Maximal model

39

57.29

Maximal model

12

24.97

- Interactions

49

62.32

- Microsatellite retained

13

24.97

- Repeat length

50

62.32

- Primer type5

14

25.46

- Unique/multiple hits

51

62.70

- Ho in source species6

17

26.91

- Microsatellite motif3

52

63.55

- No. of mismatches7

18

27.60

- No of mismatches4

53

67.65

- E-value

19

30.15

Minimal adequate model

Minimal adequate model

E-value

53

67.65

-0.02

-2.58

0.013

Repeat length

19

30.15

0.02

2.22

0.039

Microsatellite motif3

19

30.15

-1.00

-2.15

0.045

Microsatellite interrupted8

19

30.15

-1.02

-2.09

0.051


*model descriptives are given for models after removal of explanatory variables

1based on tests in three charadriiform species: Kentish plover Charadrius alexandrinus, whiskered auklet Aethia pygmaea and ruff Philomachus pugnax

2based on proportion of species in which a microsatellite locus was found to be polymorphic when tested in four individuals

3dinucleotide or tetranucleotide repeat motif of microsatellite

4total number of mismatches between chicken and charadriiform sequence for both primer sequences combined

5Gga-consensus primer or standard primer design

6observed heterozygosity for source species given in original publication of the microsatellite sequence

7number of mismatches in primer sequence between charadriiform sequence and chicken homologue

8microsatellite sequence interrupted by non-motif base insertions (imperfect) or not (perfect)

K├╝pper et al. BMC Genomics 2008 9:502   doi:10.1186/1471-2164-9-502

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