A. Depth of coverage of viruses. Depth of coverage statistics were generated for each contig (using the output of DNAStar Seqman program). Average coverage is the summed length of all sequence reads in a contig, including gaps divided by the contig length. The average and standard deviation for each virus was determined. B. Correlation of genome coverage with colonies picked. The SISPA method was performed for enterobacteriophage M13 (6407 bp), Newcastle disease virus Lasota (15,186 bp) and enterobacteriophage lambda (48502 bp). One, two or three 96-well blocks of clones were sequenced, trimmed and assembled. The sum of the total lengths of edited contigs for each condition was calculated as percent of the total reference genome length.
Djikeng et al. BMC Genomics 2008 9:5 doi:10.1186/1471-2164-9-5