Research article
A conifer genomics resource of 200,000 spruce (Picea spp.) ESTs and 6,464 high-quality, sequence-finished full-length cDNAs for Sitka spruce (Picea sitchensis)
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* Corresponding author: Jörg Bohlmann bohlmann@interchange.ubc.ca
1 Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, V6T 1Z4, Canada
2 British Columbia Cancer Agency Genome Sciences Centre, Vancouver, British Columbia, V5Z 4E6, Canada
3 Department of Botany, University of British Columbia, Vancouver, British Columbia, V6T 1Z4, Canada
4 Department of Forest Sciences, University of British Columbia, Vancouver, British Columbia, V6T 1Z4, Canada
5 Department of Biology, University of North Dakota, Grand Forks, ND, 58202-9019, USA
BMC Genomics 2008, 9:484 doi:10.1186/1471-2164-9-484
Published: 14 October 2008Additional files
Additional File 1:
cDNA library summary statistics. Sequencing statistics organized by cDNA library source for spruce expressed sequence tags.
Format: DOC Size: 53KB Download file
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Additional File 2:
Full-length cDNA inventory. Predicted protein-coding features, annotation, and GenBank accession numbers for the Sitka spruce full-length cDNA collection.
Format: XLS Size: 8.2MB Download file
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Additional File 3:
3'-end EST inventory. Detailed annotation and GenBank accession numbers for the complete set of spruce 3'-end ESTs.
Format: ZIP Size: 72.7MB Download file
Additional File 4:
5'-end EST inventory. Detailed annotation and GenBank accession numbers for the complete set of spruce 5'-end ESTs.
Format: ZIP Size: 19.2MB Download file
Additional File 5:
PUT inventory. Detailed annotation for the complete set of putative unique transcripts.
Format: ZIP Size: 24.9MB Download file
Additional File 6:
Supplemental methods. Detailed methods for RNA isolation, full-length cDNA library construction, bacterial transformation with plasmids, clone handling, DNA purification and evaluation, and DNA sequencing.
Format: DOC Size: 58KB Download file
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