Effect of ENU on the survival of msh6-/- males. Msh6-/- male rats show an increased mortality after treatment with different concentrations of ENU compared to untreated msh6-/- male rats. Survival of untreated and ENU-treated wild type rats is 100% in the indicated time-period (data not shown). Red arrowheads indicate the time points of the last ENU injection and the start of mating for F1 progeny that can be screened for mutations without risk for chimaeric progeny.
van Boxtel et al. BMC Genomics 2008 9:460 doi:10.1186/1471-2164-9-460