Table 2

Comparison of the differential expression ratios observed between embryonic stages using amplified and unamplified material. The expression ratios observed with amplified targets (IVT and PCR) come from the array datasets presented in this study. Those originating from Real-Time PCR come from the gene specific validations (IFN-tau, Cox2) we performed on unamplified RNA from each stage. Those originating from Northern blots (c12, c93, PAG11) come from previous results on unamplified RNA from each stage [26].

Array

Northern

Real-time PCR

IVT amplified material

PCR amplified material

(mRNA/18S)

(mRNA/Gapdh)

Gene

ov/tub

tub/fil

ov/fil

ov/tub

tub/fil

ov/fil

ov/tub

tub/fil

ov/fil

ov/tub

tub/fil

ov/fil

IFN-tau

-3,21

-1,57

-6,94

-2,4

-2,31

-5,55

-5,48

-1,57

-8,63

Cox2

-1,72

-1,26

-2,17

-1,22

-1,73

-2,10

-4,32

-1,74

-7,53

c12

4,93

3,86

8,51

1,87

-1,26

1,48

3,24

2,63

4,02

c93

-1,2

-1,83

-2,19

-1,66

-1,62

-2,67

-1,52

-2,87

-4,37

PAG11

-1,52

-2,87

-4,37

-1,49

-1,06

-1,58

-2,98

-1,3

-3,87

Degrelle et al. BMC Genomics 2008 9:46   doi:10.1186/1471-2164-9-46