Figure 1.

Schematic illustration of the LongSAGE-Solexa procedure. From left to right is shown the initial SAGE procedure ([6]), the improvements brought by the DeepSAGE procedure ([14]) in terms of a simpler protocol and of depth of sampling. On the right is reported the LongSAGE-Solexa procedure described in this study, which provided a major improvement along those two lines. One has to note that in the LongSAGE-Solexa procedure, a single tag is sequenced for each sequenced molecule.