Table 2

Transcript level comparison of S. aureus genes between real-time PCR and microarray analyses.

mRNA level change with microarray

mRNA level change with real-time PCR


Gene

Fold change

Fold change

Sense primer sequence

Antisense primer sequence


20 min

60 min

20 min

60 min


SA0423

8.1

19.4

9.1(± 1.4)

18.6(± 1.2)

5'-CGG GTG AAT CAG TGT GGG CAA TTT-3'

5'-TAT GAT CCG CCA CCT GAG TTC GTT-3'

SA1164

-8.9

-3.4

-40.9(± 5.0)

-2.1(± 0.1)

5'-TAT GAT CCG CCA CCT GAG TTC GTT-3'

5'-GAG TGT AGC AGG TGG TAT TCC GAT-3'

SA1225

-54.6

-7.7

-265.0(± 18.9)

-58.0(± 1.4)

5'-GAG TGT AGC AGG TGG TAT TCC GAT-3'

5'-TCA TCA GTT GGA TCC GCT TCA GCA-3'

SA1226

-21.5

-4.3

-14.9(± 3.3)

-2.1(± 0.7)

5'-TCA TCA GTT GGA TCC GCT TCA GCA-3'

5'-ACT TTA GGC AGA GGC GGT TCT GAT-3'

SA1227

-27.3

-5.2

-58.0(± 1.5)

-2.8(± 0.1)

5'-ACT TTA GGC AGA GGC GGT TCT GAT-3'

5'-AGT CTT GGG TCA GTG GCA TAC ACA-3'

SA1228

-31.4

-5.1

-35.3(± 1.6)

-1.6(± 0.4)

5'-AGT CTT GGG TCA GTG GCA TAC ACA-3'

5'-TGG GTG CAA CAG GAT TAG TAG GCA-3'

SA1229

-23.5

-4.4

-7.4(± 2.0)

-1.2(± 0.2)

5'-TGG GTG CAA CAG GAT TAG TAG GCA-3'

5'-TTC AAC TTC TTG CCC TGC AGA ACG-3'

SA2093

6.8

9.0

15.7(± 1.2)

4.6(± 0.5)

5'-TTC AAC TTC TTG CCC TGC AGA ACG-3'

5'-TAT TTG AGG GTG TTG GCG TTG CAC-3'

SA2097

6.9

11.5

5.5(± 1.8)

24.5(± 1.2)

5'-TAT TTG AGG GTG TTG GCG TTG CAC-3'

5'-AGG GCT CTC AGC AGT AGT TCC ATT-3'

SA2353

10.2

13.8

8.0(± 1.6)

16.4(± 1.9)

5'-AGG GCT CTC AGC AGT AGT TCC ATT-3'

5'-ATT CGT GGA GGT ACG ATT GTC GGT-3'

SA2355

8.5

9.4

5.3(± 1.3)

20.1(± 1.7)

5'-ATT CGT GGA GGT ACG ATT GTC GGT-3'

5'-GCT GCT TGT ATA GCA CCA TTC GCA-3'

SA2459c

-2.5

2.5

-3.1(± 0.2.)

2.0(± 0.3)

5'-TTG TCG ACG TTG GCT ACT GGG ATA-3'

5'-TGG AAC CAA CAT CCA ACA CAT GGC-3'

SA2460c

-2.0

4.7

-1.8(± 0.3)

2.5(± 0.3)

5'-ATG GTC AAG CCC AGA CAG AGG GAA TA-3'

5'-CAC ACG ATA TAG CGA TAA GTG CTG TT-3'

SA2461c

-2.1

3.4

-2.6(± 0.5)

2.1(± 0.5)

5'-AGC AGT CAC TCC GAA CTC CAA TGA-3'

5'-TCA TGG AAT CCG TCC CAT CTC T-3'

SA2464b

-10.5

-

-5.5(± 1.4)

-

5'-GCT GCT TGT ATA GCA CCA TTC GCA-3'

5'-GAT CGT CGC AAT TCT GCC ATT CCA-3'

SA2465b

-10.6

-

-13.0(± 1.4)

-

5'-GAT CGT CGC AAT TCT GCC ATT CCA-3'

5'-TTG TTG CGC CCA TCA TAA CGA CAG-3'

SA2466b

-14.5

-

-5.7(± 1.3)

-

5'-TTG TTG CGC CCA TCA TAA CGA CAG-3'

5'-ACC GTA CTG GTG GTT TAG GTG CAA-3'

SA2468b

-13.6

-

-58.1(± 1.3)

-

5'-ACC GTA CTG GTG GTT TAG GTG CAA-3'

5'-TGA ACG GCC ATT TGA TGA TGG AGC-3'

SA0727a

1.00

1.00

1.00

1.00

5'-GAT GGT GGT TTC CGC GTA AAT GGT-3'

5'-GCG CCT GCT TCA ATA TGA GCT TGT-3'


The real time PCR results are the mean of three biological replicates with three technical replicates for each gene. The microarray results are the mean of five replicates of each gene.

aSA0727 was glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and used as the house-keeping gene. bSA2464, SA2465, SA2466, and SA2468 were downregulated at 20 min with no change at 60 min. cSA2459, SA2460, and SA2461 were downregulated after 20 min and upregulated after 60 min exposure.

Jang et al. BMC Genomics 2008 9:411   doi:10.1186/1471-2164-9-411

Open Data