Table 2

Bacterial strains and plasmids used in this study

Strain or plasmid

Relevant characteristics

Source or reference


Strains


S. oneidensis

MR-1 (ATCC 700550)

Wild type

Laboratory Stock

MR-1/Δso2426

In-frame deletion of the so2426 locus

This study

MR-1/Δso2426(so2426+)

Δso2426 mutant complemented with pBBRso2426

This study

E. coli WM3064

DAP-auxotrophic wild type

[32]


Plasmids


pJK100

Allelic-exchange vector

[32]

pJK102

pJK100 with so2426 upstream and downstream regions

This study

pCM157

Cre recombinase expression vector

[32]

pBBR1MCS-5

Gmr, broad-host range vector, lacPOZ'

[56]

pBBRso2426

pBBR1MCS5-based construct harboring entire so2426 gene

This study


Chourey et al. BMC Genomics 2008 9:395   doi:10.1186/1471-2164-9-395

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