Relative siderophore production by wild-type MR-1 and Δso2426 mutant cells under chromate conditions. A final chromate concentration of 0.3 mM was added to mid-log-phase (OD600, 0.5) wild-type MR-1 (●) and Δso2426 mutant (○) cultures cultivated in LB broth, and relative siderophore synthesis by each culture was monitored over time using the CAS assay. The relative siderophore production was calculated by subtracting the supernatant A630 for the wild type or mutant from the uninoculated control and then determining the ratio of corrected supernatant A630 to control A630. The graphed values represent mean ratios ± standard errors (bars) for three replicate CAS measurements.
Chourey et al. BMC Genomics 2008 9:395 doi:10.1186/1471-2164-9-395