Table 2

Differentially expressed chaperone and chaperone-related genes in artesunate-treated cells

Gene ID

Description

Pexel

family

ref

ART 90 mn

ART 3 h

HS

CQ


PFE1605w

DNAJ protein

yes

resa like/dnaJ

1, 3

1,01

PFD0080c

hypothetical protein, conserved in P.falciparum

yes

resa like

3

1,31

1,82

+

PF10_0378

DnaJ protein, putative

yes

Hsp40, type III

1, 2, 4

1,33

1,53

+

PFE0040c

mature parasite-infected erythrocyte surface antigen (MESA) or PfEMP2

yes

Hsp40, type IV

2, 4

0,92

1,87

PFA0660w

protein with DNAJ domain, dnj1/sis1 family

yes

Hsp40, type II

1, 2, 4

1,01

1,34

+

MAL7P1.7

RESA-like protein

yes

resa like

3

0,83

PFL0050c

hypothetical protein

yes

resa like

3

0,86

PF07_0029

heat shock protein 86

no

hsp90

1

0,9

+

PF07_0030

heat shock protein 86 family protein

no

Hsp86 family

5

1,63

+

PF08_0054

heat shock 70 kDa protein

no

hsp70

1

0,88

+

PF11_0034

hypothetical protein

yes

Hsp40, type IV

1, 2, 4

0,81

+

PFA0675w

P. falciparum RESA-like protein with DnaJ domain

yes

Hsp40, type IV

1, 2, 4

1,96

+

PF14_0700

hypothetical protein, conserved

no

Hsp40, type III

1, 2, 4

0,81

PF11_0509

ring-infected erythrocyte surface antigen putative

yes

Hsp40, type IV

1, 2, 4

-1,14

PF13_0102

DNAJ-like Sec63 homologue

no

Hsp40, type III

1, 2, 4

-1,04

PF11_0216

hypothetical protein

no

Heat shock factor binding protein 1

6

-0,93

-

PFC0975c

PFCYP19, cyclophilin, peptidyl-prolyl cis-trans isomerase

no

cyclophillin

1

-0,97


Gene ID: identifier as found in PlasmoDB.

Art columns: log ratios of gene expression under artesunate exposure at 90 minutes and 3 hours.

HS column: over (+) or under (-) expression.

Results were compared with transcriptome modifications induced by heat-shock (HS) [12] and chloroquine (CQ) [8].

Chaperone- and chaperone-related genes were defined based on: 1) Acharya et al. [19], 2) Botha et al. [20], 3) resa-like annotation from GeneDB, 4) genes fitting the HMM profile of DNAJ (PF00226) from Pfam, 5) The Hsp86 family annotation from GeneDB, 6) genes fitting the HMM profile of heat shock factor binding protein 1 (PF06825) from Pfam.

Natalang et al. BMC Genomics 2008 9:388   doi:10.1186/1471-2164-9-388

Open Data