Table 2

Weight, oviposition and hatching of replete R. microplus female ticks after RNAi.

Experimental group

Tick weight (mg)a

Eggs per tick (mg)b

Hatching rate (%)c

Expression silencing (%)d


Saline control

318 (272–367)

173 (128–229)

> 90

---

GI

318 (260–369)

123 (31–175)

> 90

55 ± 8**

GII

315 (267–349)

132 (95–182)

0.2*

84 ± 5**

Subolesin

318 (270–360)

142 (101–175)

0.4*

46 ± 8**


aReplete R. microplus ticks were collected and weighed individually before injection with dsRNA, all within 6 hours after dropping of the host. The average weight and variation (between parentheses) of each group are shown. No significant statistical differences were observed (P > 0.05).

bThe egg mass oviposited by each tick was weighed individually. The average egg mass and variation (between parentheses) was calculated and compared with the saline-injected control ticks by Student's t-test with unequal variance. No significant statistical differences were observed (P > 0.05).

cThe hatching rate was determined 6 weeks post oviposition and compared with the saline-injected control ticks by Student's t-test with unequal variance (*P < 0.01).

dThe expression silencing of target genes was determined in eggs by real-time RT-PCR and average ± S.D. mRNA levels calculated and compared between dsRNA and saline-injected control ticks by Student's t-test with unequal variance (**P < 0.05). Amplification efficiencies were normalized against β-actin.

de la Fuente et al. BMC Genomics 2008 9:372   doi:10.1186/1471-2164-9-372

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