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Weight, oviposition and hatching of replete R. microplus female ticks after RNAi. |
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| Experimental group |
Tick weight (mg)a |
Eggs per tick (mg)b |
Hatching rate (%)c |
Expression silencing (%)d |
|
|
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| Saline control |
318 (272–367) |
173 (128–229) |
> 90 |
--- |
| GI |
318 (260–369) |
123 (31–175) |
> 90 |
55 ± 8** |
| GII |
315 (267–349) |
132 (95–182) |
0.2* |
84 ± 5** |
| Subolesin |
318 (270–360) |
142 (101–175) |
0.4* |
46 ± 8** |
|
aReplete R. microplus ticks were collected and weighed individually before injection with dsRNA, all within 6 hours after dropping of the host. The average weight and variation (between parentheses) of each group are shown. No significant statistical differences were observed (P > 0.05). bThe egg mass oviposited by each tick was weighed individually. The average egg mass and variation (between parentheses) was calculated and compared with the saline-injected control ticks by Student's t-test with unequal variance. No significant statistical differences were observed (P > 0.05). cThe hatching rate was determined 6 weeks post oviposition and compared with the saline-injected control ticks by Student's t-test with unequal variance (*P < 0.01). dThe expression silencing of target genes was determined in eggs by real-time RT-PCR and average ± S.D. mRNA levels calculated and compared between dsRNA and saline-injected control ticks by Student's t-test with unequal variance (**P < 0.05). Amplification efficiencies were normalized against β-actin. | ||||
de la Fuente et al. BMC Genomics 2008 9:372 doi:10.1186/1471-2164-9-372 |
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