Evidence of the role of tick subolesin in gene expression

doi:10.1186/1471-2164-9-372

BMC Genomics

Volume 9

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de la Fuente J
Maritz-Olivier C
Naranjo V
Ayoubi P
Nijhof AM
Almazán C
Canales M
de la Lastra JM
Galindo RC
Blouin EF
Gortazar C
Jongejan F
Kocan KM

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de la Fuente J
Maritz-Olivier C
Naranjo V
Ayoubi P
Nijhof AM
Almazán C
Canales M
de la Lastra JM
Galindo RC
Blouin EF
Gortazar C
Jongejan F
Kocan KM
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Research article

Evidence of the role of tick subolesin in gene expression

José de la Fuente¹^,2 , Christine Maritz-Olivier³ , Victoria Naranjo² , Patricia Ayoubi⁴ , Ard M Nijhof⁵ , Consuelo Almazán⁶ , Mario Canales² , José M Pérez de la Lastra² , Ruth C Galindo² , Edmour F Blouin¹ , Christian Gortazar² , Frans Jongejan⁵^,7 and Katherine M Kocan¹

¹Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA

²Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM), Ronda de Toledo s/n, 13071 Ciudad Real, Spain

³Department of Biochemistry, Faculty of Natural and Agricultural Sciences, Agriculture Building, Lunnon Road, University of Pretoria, Pretoria, 0002, South Africa

⁴Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA

⁵Utrecht Centre for Tick-borne Diseases (UCTD), Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584CL, Utrecht, The Netherlands

⁶Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Tamaulipas, Km. 5 carretera Victoria-Mante, CP 87000 Cd. Victoria, Tamaulipas, Mexico

⁷Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, 0110, Onderstepoort, South Africa

author email corresponding author email

BMC Genomics 2008, 9:372doi:10.1186/1471-2164-9-372


Published:	2 August 2008

Abstract

Background

Subolesin is an evolutionary conserved protein that was discovered recently in Ixodes scapularis as a tick protective antigen and has a role in tick blood digestion, reproduction and development. In other organisms, subolesin orthologs may be involved in the control of developmental processes. Because of the profound effect of subolesin knockdown in ticks and other organisms, we hypothesized that subolesin plays a role in gene expression, and therefore affects multiple cellular processes. The objective of this study was to provide evidence for the role of subolesin in gene expression.

Results

Two subolesin-interacting proteins were identified and characterized by yeast two-hybrid screen, co-affinity purification and RNA interference (RNAi). The effect of subolesin knockdown on the tick gene expression pattern was characterized by microarray analysis and demonstrated that subolesin RNAi affects the expression of genes involved in multiple cellular pathways. The analysis of subolesin and interacting protein sequences identified regulatory motifs and predicted the presence of conserved protein kinase C (PKC) phosphorylation sites.

Conclusion

Collectively, these results provide evidence that subolesin plays a role in gene expression in ticks.