Gene networks driving bovine milk fat synthesis during the lactation cycle

Additional file 1:

Supplementary Materials and Methods and Results and Discussion. The file contain additional Materials and Methods (RNA extraction, PCR, and design and evaluation of primers and details about measurement of milk yield, milk composition and fatty acid analysis) accompanied by 4 tables which include cows features (Table S1), qPCR primers information (Table S2) and validation (Tables S3 and S4); the file contain also additional Results and Discussion about milk FA composition, accompanied by 3 tables (Table S5 for PCR features, Table S6 for mole/day and Table S7 for g/100 g of milk fatty acids with calculated indexes) and 6 figures which include: the entire curve of lactation and milk fat yield and % (Figure S1), Δ⁵ and Δ⁶ calculated indexes (Figure S2), possible model of utilization of BHBA in bovine mammary (Figure S3); possible utilization of BHBA for mitochondrial FA synthesis (Figure S4), and possible model of phospholipid metabolism in bovine mammary during lactation (Figure S5). For each figure a detailed discussion is also provided in the caption.

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Additional file 2:

Excel file with correlation. The file contains Pearson correlations among all measurements (genes and FA yield – mole/day). Correlations were analyzed using PROC CORR of SAS (SAS Inst. Inc. Cary, NC, release 8.0). The file contains 3 sheets with correlations and a legend.

Format: XLS Size: 475KB Download file

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