BMC Genomics

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Open Access Highly Access Research article

Analysis of expressed sequence tags from Actinidia: applications of a cross species EST database for gene discovery in the areas of flavor, health, color and ripening

Ross N Crowhurst1, Andrew P Gleave1, Elspeth A MacRae1, Charles Ampomah-Dwamena1, Ross G Atkinson1, Lesley L Beuning1, Sean M Bulley1, David Chagne1, Ken B Marsh1, Adam J Matich1, Mirco Montefiori1, Richard D Newcomb1, Robert J Schaffer1, Björn Usadel2, Andrew C Allan1, Helen L Boldingh1, Judith H Bowen1, Marcus W Davy1, Rheinhart Eckloff1, A Ross Ferguson1, Lena G Fraser1, Emma Gera1, Roger P Hellens1, Bart J Janssen1, Karin Klages1, Kim R Lo1, Robin M MacDiarmid1, Bhawana Nain1, Mark A McNeilage1, Maysoon Rassam1, Annette C Richardson1, Erik HA Rikkerink1, Gavin S Ross1, Roswitha Schröder1, Kimberley C Snowden1, Edwige JF Souleyre1, Matt D Templeton1, Eric F Walton1, Daisy Wang1, Mindy Y Wang1, Yanming Y Wang1, Marion Wood1, Rongmei Wu1, Yar-Khing Yauk1 and William A Laing1*

Author Affiliations

1 The Horticultural and Food Research Institute of New Zealand, PB 92169, Auckland, New Zealand

2 Max-Planck-Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany

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BMC Genomics 2008, 9:351 doi:10.1186/1471-2164-9-351

Published: 27 July 2008

Additional files

Additional file 1:

Additional Table 1. Expressed sequence tags (ESTs) sequenced in Angiosperm orders and families.

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Additional file 2:

Additional Table 2a. Summary of library names, descriptions and statistics for sequenced Actinidia ESTs. Additional Table 2b. Actinidia libraries that were subtracted before sequencing to reduce redundant sequences.

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Additional file 3:

Additional Table 3 Codon usage calculated using 707 full-length Actinidia cDNA sequences.

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Additional file 4:

Additional Table 4. Source of oligonucleotides used to construct the Actinidia microarray slides by library and species

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Additional file 5:

Additional Table 5. Highly expressed ESTs in the Actinidia EST database.

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Additional file 6:

Additional Table 6. Most frequent InterPro families found in Actinidia NRs.

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Additional file 7:

Additional Table 7. Volatiles, acids, and sugars in Actinidia flowers and fruit

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