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Open AccessResearch article

Gene expression patterns of sulfur starvation in Synechocystis sp. PCC 6803

Zhigang Zhang1,2 email, Ninad D Pendse1,2,5 email, Katherine N Phillips3 email, James B Cotner3 email and Arkady Khodursky1,4 email

1BioTechnology Institute, 1479 Gortner Avenue, University of Minnesota, St. Paul, MN 55108, USA

2Department of Chemical Engineering and Materials Science, 421 Washington Avenue SE, University of Minnesota, Minneapolis, MN 55455, USA

3Department of Ecology, Evolution and Behavior, 1987 Upper Buford Circle, University of Minnesota, St. Paul, MN 55108, USA

4Department of Biochemistry, Molecular Biology and Biophysics, 1479 Gortner Avenue, University of Minnesota, St. Paul, MN 55108, USA

5InVivoScribe Technology, 6330 Nancy Ridge Drive, Suite 106, San Diego, CA 92121, USA

author email corresponding author email

BMC Genomics 2008, 9:344doi:10.1186/1471-2164-9-344

Published: 21 July 2008

Additional files

Additional File 1:

microarray_construction. This supplementary text describes the oligonucleotide probe design and microarray construction and validation.

Format: DOC Size: 1.9MB Download file

This file can be viewed with: Microsoft Word Viewer

Additional File 2:

genesets. This file contains gene set annotations assembled from Cyanobase.

Format: XLS Size: 70KB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional File 3:

s_deprivation_genes. A complete list of genes significantly affected by S deprivation. A statistical significance threshold was set at 1% false discovery rate (FDR). Top ranking differentially expressed genes were selected based on one additional criterion: at least 50% change in mean transcript abundance in at least 1 time point along the time course. The genes were listed based on the order, from top to bottom, in the clustered heat-map shown in Figure 2. Functional sub-/categories, clustering, gene annotations and log2(ratio) values are listed.

Format: XLS Size: 86KB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional File 4:

supplementary_tables_figures. This file contains supplementary tables and figures.

Format: DOC Size: 311KB Download file

This file can be viewed with: Microsoft Word Viewer

Additional File 5:

synecho_oligo_sequences. This file contains all of the array oligo probe sequences (sheet 1), including 3064 for Synechocystis ORFs, 13 for E. coli K-12 ORF controls, and 3 for Synechocystis rRNA controls. 104 genes that we failed to design oligo-probes for, and that were excluded from our microarray platform, are listed in sheet 2.

Format: XLS Size: 480KB Download file

This file can be viewed with: Microsoft Excel Viewer


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