Table 5

Comparison of fold-change results on the discordant genes between SYBR Green PCR Arrays and other platforms

RT2 Profiler PCR Array

TaqMan

StaRT-PCR

QuantiGene

AFX

ABI

AG1

GEH

ILM


FURIN

1.16

    -43.98

-1.49

-1.42

-1.21

1.04

-1.38

N/A

-1.12

RB1

1.73*

-1.92

-1.59

    -3.09

    -2.23

-1.30

    -4.06

    -2.14

    -2.14

ABCD1

-5.44

-7.11

N/A

-3.83

-1.45

    2.21

-8.25

    2.15

-2.41

BAG1

1.66

-1.06

1.85

-1.97

    -2.89

-1.00

1.12

-1.01

1.18

BAG4

2.66

1.01

N/A

1.96

1.30

    -2.25

    -2.25

1.13

1.51

CDK5R1

45.53

28.92

N/A

21.55

18.11

    -1.09

1.56

2.11

14.26

IGFBP5

-1.68

-2.69

-1.98

-2.16

-2.68

    4.11

-3.26

-2.42

-2.37

JAK2

2.11

1.31

N/A

1.25

1.06

1.11

    -1.15

1.99

1.06


Fold-change values for the above platforms other than RT2 Profiler PCR Arrays are derived from the data provided by MAQC studies [8,12]. A positive value for fold change indicates a higher expression while a negative value indicates a lower expression in Sample B when compared with Sample A. "N/A" means the gene was not included in the indicated platform. Discordant fold-change values compared to RT2 PCR Arrays are underlined and in bold. *The fold-change value for RB1 gene from the PCR Array using the corrected sequence is -1.75 (see text for details).

Arikawa et al. BMC Genomics 2008 9:328   doi:10.1186/1471-2164-9-328

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