Additional file 11.
Supplemental Figure 3. Confirmation of microarray data by sqRT-PCR and Northern hybridizations. Panel A shows confirmation of microarray data using sqRT-PCR for genes induced in PPV-infected Arabidopsis protoplasts. Panel B shows confirmation of microarray data using sqRT-PCR (left panel) and Northern hybridizations (right panel) for Arabidopsis genes differentially regulated in PPV-infected protoplasts and in PPV-infected leaves. Probes for sqRT-PCR and Northern hybridizations were generated by PCR amplification of Arabidopsis cDNA using gene specific primers shown in Table 3. sqRT-PCR of the constitutively expressed Actin 2 gene (At3g18780) was used as a loading control. pPPV-SK68, a PPV infectious cDNA clone used to transfect protoplasts; pPPV-SK68Δ, a mutant non-infectious clone of pPPV-SK68 was used as a control; hpt, hours post transfection.
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Babu et al. BMC Genomics 2008 9:325 doi:10.1186/1471-2164-9-325