Table 5

Validation of SNPs with conventional sequencing.

Amplified contig

Non-validated

Validated

Number of predicted SNPs


amplicon01

KIRST.1015.C2

4 (44%)

5 (56%)

9

amplicon02

KIRST.2351.C2

4 (44%)

5 (56%)

9

amplicon03

KIRST.1461.C1

2 (40%)

3 (60%)

5

amplicon04

KIRST.1992.C1

3 (38%)

5 (63%)

8

amplicon05

KIRST.25.C1

4 (36%)

7 (64%)

11

amplicon06

KIRST.1936.C1

3 (33%)

6 (67%)

9

amplicon07

KIRST.12521.C1

2 (33%)

4 (67%)

6

amplicon08

KIRST.15421.C1

2 (33%)

4 (67%)

6

amplicon09

KIRST.2632.C1

1 (33%)

2 (67%)

3

amplicon10

KIRST.4036.C2

4 (33%)

8 (67%)

12

amplicon11

KIRST.5530.C1

2 (33%)

4 (67%)

6

amplicon12

KIRST.3079.C1

2 (29%)

5 (71%)

7

amplicon13

KIRST.15389.C1

1 (25%)

3 (75%)

4

amplicon14

KIRST.486.C3

2 (25%)

6 (75%)

8

amplicon15

KIRST.823.C1

2 (22%)

7 (78%)

9

amplicon16

KIRST.854.C4

2 (20%)

8 (80%)

10

amplicon17

KIRST.2687.C1

1 (20%)

4 (80%)

5

amplicon18

KIRST.4822.C1

1 (20%)

4 (80%)

5

amplicon19

KIRST.340.C6

2 (18%)

9 (82%)

11

amplicon20

KIRST.11157.C1

1 (17%)

5 (83%)

6

amplicon21

KIRST.152.C3

1 (17%)

5 (83%)

6

amplicon22

KIRST.8182.C6

1 (17%)

5 (83%)

6

amplicon23

KIRST.1003.C1

1 (14%)

6 (86%)

7

amplicon24

KIRST.1268.C4

1 (14%)

6 (86%)

7

amplicon25

KIRST.1975.C1

1 (14%)

6 (86%)

7

amplicon26

KIRST.4785.C1

1 (14%)

6 (86%)

7

amplicon27

KIRST.52.C8

1 (14%)

6 (86%)

7

amplicon28

KIRST.340.C1

2 (13%)

13 (87%)

15

amplicon29

KIRST.17053.C1

1 (13%)

7 (88%)

8

amplicon30

KIRST.52.C1

1 (13%)

7 (88%)

8

amplicon31

KIRST.8655.C1

1 (8%)

11 (92%)

12

amplicon32

KIRST.1285.C3

1 (7%)

14 (93%)

15

amplicon33

KIRST.1441.C5

0 (0%)

5 (100%)

5

amplicon34

KIRST.17202.C1

0 (0%)

8 (100%)

8

amplicon35

KIRST.2273.C1

0 (0%)

7 (100%)

7

amplicon36

KIRST.2790.C1

0 (0%)

11 (100%)

11

amplicon37

KIRST.2900.C3

0 (0%)

4 (100%)

4

amplicon38

KIRST.34.C15

0 (0%)

8 (100%)

8

amplicon39

KIRST.344.C1

0 (0%)

7 (100%)

7

amplicon40

KIRST.4650.C2

0 (0%)

7 (100%)

7

amplicon41

KIRST.5060.C2

0 (0%)

10 (100%)

10

amplicon42

KIRST.5120.C1

0 (0%)

10 (100%)

10

amplicon43

KIRST.6233.C1

0 (0%)

6 (100%)

6


Total

43 contigs

58 (17%)

279 (83%)

337


Number and percentage of non-validated and validated SNPs for each of the 43 amplicons sequenced with dideoxy-based method

Novaes et al. BMC Genomics 2008 9:312   doi:10.1186/1471-2164-9-312

Open Data