Table 2

Summary and distribution of assembled sequences. Length distribution and characteristics of contigs assembled from the two GS-20 runs, one GS-FLX run, the three 454 runs combined and from the control Sanger sequenced ESTs

GS-20

GS-FLX

454 all

Sanger (control)


Run(s) in assembly

1 + 2

3

1 + 2 + 3

-


≤ 100 bp

18987 (29%)

52 (<1%)

10820 (15%)

0

101 – 250 bp

42320 (66%)

17348 (62%)

35958 (50%)

0

251 – 500 bp

2476 (4%)

5355 (19%)

18768 (26%)

7564 (35%)

501 – 750 bp

535 (<1%)

2463 (9%)

2869 (4%)

9226 (43%)

751 – 1000 bp

167 (<1%)

1314 (5%)

1396 (2%)

2830 (13%)

> 1000 bp

99 (<1%)

1547 (6%)

1573 (2%)

1812 (8%)


Total contigs

64584 (100%)

28079 (100%)

71384 (100%)

21432 (100%)

Average contig length (bp)

130.6

353.22

247.16

623.35

Reads in contigs

80.72%

71.36%

88.41%

84,88%

Average reads/contig

7.89

9.98

12.69

3.42


Novaes et al. BMC Genomics 2008 9:312   doi:10.1186/1471-2164-9-312

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