Genetic marker anchoring by six-dimensional pools for development of a soybean physical map

doi:10.1186/1471-2164-9-28

BMC Genomics

Volume 9

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Research article

Genetic marker anchoring by six-dimensional pools for development of a soybean physical map

Xiaolei Wu¹ , Guohua Zhong¹ , Seth D Findley¹ , Perry Cregan² , Gary Stacey¹^,3 and Henry T Nguyen¹

¹Division of Plant Sciences and National Center for Soybean Biotechnology, University of Missouri-Columbia, Columbia, MO 65211, USA

²Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA

³Department of Biochemistry; Department of Molecular Microbiology and Immunology, University of Missouri-Columbia, Columbia, MO 65211, USA

author email corresponding author email

BMC Genomics 2008, 9:28doi:10.1186/1471-2164-9-28


Published:	22 January 2008

Additional files

Additional file 1:

PCR screening workflow. The workflow for PCR screening of 6-D pools is illustrated.

Format: PDF Size: 10KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional file 2:

The complete list of mapped markers using 6D pools. The data provided included all information for the 1541 markers about marker name, primer sequence, linkage group, BAC clones targeted and FPC contig number.

Format: XLS Size: 1.3MB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional file 3:

Placement of markers and BES of the 8 cM-region on LG E. The dataset shows the placements of 36 markers and BES of 193 clones located in the 8 cM-region on LG E onto the 7× soybean assembly.

Format: XLS Size: 216KB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional file 4:

Summary of BES anchoring to 7× assembly. The data is summarized to show the percentage of BAC clones anchored to the 7× assembly based on the placements of markers and BES in additional file 3.

Format: XLS Size: 15KB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional file 5:

Annotation of unigenes mapped by in silico approach. The data provided the information about annotation of unigenes mapped by in silico approach and the number of BAC hits.

Format: XLS Size: 621KB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional file 6:

The complete blast list of in silico mapping. The dataset shows the results of blasting soybean unigenes against BES of BstY I BAC library.

Format: XLS Size: 2.2MB Download file

This file can be viewed with: Microsoft Excel Viewer