Research article
Smoking-induced gene expression changes in the bronchial airway are reflected in nasal and buccal epithelium
- † Equal contributors
1 Pulmonary Center, Boston University School of Medicine, Albany Street, Boston Massachusetts, USA
2 Pathology Program, Graduate Medical Sciences, Boston University School of Medicine, Albany Street, Boston Massachusetts, USA
3 Department of Genetics and Genomics, Boston University School of Medicine, Albany Street, Boston, MA, USA
4 Bioinformatics Program, Boston University School of Engineering, Cummington Street, Boston Massachusetts, USA
BMC Genomics 2008, 9:259 doi:10.1186/1471-2164-9-259
Published: 30 May 2008Additional files
Additional File 1:
Additional Methods. Information provided represents further methodology for Gene Set Enrichment Analysis comparing expression of genes in buccal, nasal, and bronchial epithelial datasets, as well as methodology for real-competitive PCR analysis of buccal mucosa gene expression.
Format: DOC Size: 38KB Download file
This file can be viewed with: Microsoft Word Viewer
Additional File 2:
Gene Set Enrichment Analysis Strategy. Strategic flow for Gene Set Enrichment Analysis to determine the distribution of genes differentially expressed in the bronchial epithelium of smokers within the ranked list of gene expression differences observed between smokers and non-smokers in buccal and nasal epithelial samples.
Format: PDF Size: 164KB Download file
This file can be viewed with: Adobe Acrobat Reader
Additional File 3:
Figure legend for Gene Set Enrichment Analysis Strategy. Information provided represents the figure legend for Additional Figure 1, explaining the strategic flow of gene set enrichment analysis described in the main manuscript.
Format: DOC Size: 24KB Download file
This file can be viewed with: Microsoft Word Viewer
Additional File 4:
Subject demographics for real competitive PCR studies. Data provided represents demographics for buccal mucosa samples used in the real competitive PCR studies.
Format: DOC Size: 27KB Download file
This file can be viewed with: Microsoft Word Viewer
Additional File 5:
Differential expression of overlapping leading edge genes. Data provided represents fold change and p-values for the 5 most differentially expressed genes in the nose or mouth among the 45 genes induced by smoking in the bronchial airway that are present in both the nasal and buccal "leading edge subsets"
Format: DOC Size: 34KB Download file
This file can be viewed with: Microsoft Word Viewer
Additional File 6:
Real competitive PCR results. Data provided represents gene expression and fold change for three genes validated in additional buccal mucosa samples from Additional File 4 via real competitive PCR.
Format: DOC Size: 26KB Download file
This file can be viewed with: Microsoft Word Viewer
