Validation of oligoarrays for quantitative exploration of the transcriptome1Department of Tumor Biology, Institute for Cancer Research, Norwegian Radium Hospital, Montebello, Oslo, Norway 2PubGene AS, Vinderen, Oslo, Norway 3Norwegian Computing Center, Oslo, Norway 4Department of Developmental Biology, Harvard School of Dental Medicine, Boston, MA, USA 5Laboratory for Innovative Translational Technologies, Harvard School of Dental Medicine, Boston, MA, USA 6Decision Systems Group, Brigham and Women's Hospital, Boston, MA, USA 7Department of Genetics, Harvard Medical School, Boston, MA, USA 8Howard Hughes Medical Institute, Harvard Medical School, Boston, MA, USA 9Department of Biostatistics, Institute of Basic Medical Sciences, University of Oslo, Norway 10Department of Mathematics, University of Oslo, Norway 11Department of Mathematics, Vrije Universiteit Amsterdam, The Netherlands 12Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands 13Department of Medical Informatics, Institute for Cancer Research, Norwegian Radium Hospital, Montebello, Oslo, Norway 14Department of Radiation Biology, Institute for Cancer Research, Norwegian Radium Hospital, Montebello, Norway
BMC Genomics 2008, 9:258doi:10.1186/1471-2164-9-258
Additional filesAdditional file 1: Transcript data of genes in common for oligoarrays, MPSS, and SAGE. The file lists transcript data obtained with oligoarrays (number of transcripts per μg total RNA and number of transcripts per cell), MPSS (tags per million and tags per cell) and SAGE (tags per million and tags per cell) for the overlapping genes. Format: XLS Size: 215KB Download file This file can be viewed with: Microsoft Excel Viewer Additional file 2: QRT-PCR primer sequences, The file lists the forward and reverse primer sequences and amplicon sequences for the qRT-PCR analyses. Format: XLS Size: 22KB Download file This file can be viewed with: Microsoft Excel Viewer Additional file 3: PCR amplification efficiency of selected genes. The file lists the slope and standard deviation of linear curves fitted to plots of cycle threshold (Ct) versus primer dilution for the genes subjected to qRT-PCR analysis. Format: XLS Size: 23KB Download file This file can be viewed with: Microsoft Excel Viewer |




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