Additional file 9.

Q-RT-PCR validation of microarray results across multiple culture samples. (A) Q-RT-PCR versus microarray log expression ratios (timepoint vs. 0 hour) from CD3+ T-cell activation experiments, E1–E3, (for all 12 (= 3 × 4) timepoints: 4, 10, 48 and 96 hours of 3 experiments) for each of the 8 selected genes (FOS, MYB, JUN, CAT, MAPK6, SORD, SOD2, and STAT1). (B) Q-RT-PCR versus microarray log expression ratios (timepoint vs. 0 hour) from CD4+ and CD8+ T-cell activation experiments, E8 and E9, (for all 20 (= 2 × 2 × 5) timepoints: 6, 12, 24, 48 and 72 hours of 2 experiments) for each of the 7 selected genes (EGR1, EGR2, EGR3, FASL, GZMA, GZMB, and MYB).

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Wang et al. BMC Genomics 2008 9:225   doi:10.1186/1471-2164-9-225