Figure 4.

Protein expression profiles supported the different transcription patterns between CD4+ and CD8+ subsets. CD4+ T cells and CD8+ T cells were selected, stimulated (with anti-CD3/anti-CD28 antibodies), cultured separately and harvested at the indicated timepoints of culture. Flow cytometric assays were carried out for the selected genes with different transcription patterns between CD4+ and CD8+ subsets ((A) TNFSF5, (B) TNFSF4, (C) TNFRSF9, (D) KLRD1, (E) CD48, and (F) CD69). Data from two independent CD4+ and CD8+ T-cell experiments, E8 and E9, are shown.