Table 2

Independent confirmation of the yeast two-hybrid results

Interaction in two-hybrid assay

Confirmation method

Reference

CPSF160 – CPSF100

In vitro pull-down assay; Co-purification from nuclear extracts with tagged protein

[31, 34]

CPSF100 – CPSF73-I

In vitro pull-down assay; Co-purification from nuclear extracts with tagged protein

[31, 34]

CPSF100 – CPSF73-II

In vitro pull-down assay; Co-purification from nuclear extracts with tagged protein

[31, 34]

CPSF100 – CPSF30

In vitro pull-down assay

[34]

CPSF30 – CPSF30

In vitro copurification with recombinant proteins

[27]

CPSF100 – FY

Co-purification from nuclear extracts with tagged protein

[31]

CPSF30 – FIPS5

In vitro copurification with recombinant proteins, direct effects on enzyme activity

[28, 29]

CSTF77 – CSTF64

In vitro copurification with recombinant proteins

[30]

CSTF77 – FIPS5

In vitro copurification with recombinant proteins

[29]

CFIS1 – FIPS5

In vitro copurification with recombinant proteins

[29]

FIPS5 – PABN2

In vitro copurification with recombinant proteins

[29]

FIPS5 – PAPS4

In vitro copurification with recombinant proteins, direct effects on enzyme activity

[29]

CLPS3 – PCFS1

In vitro pull-down

D. Xing and QQ. Li, unpublished data (manuscript in prep)

CLPS3 –PCFS5

In vitro pull-down

D. Xing and QQ. Li, unpublished data (manuscript in prep)

CLPS3 – PCFS4

Affinity co-purification

[39]

Hunt et al. BMC Genomics 2008 9:220   doi:10.1186/1471-2164-9-220