Figure 3.

The 130 bp amplicon is reliably amplified from old museum specimens. Example here is from the species of the Lepidoptera genus Coleophora. The tree is reconstructed using neighbor-joining method [12] with Kimura two-parameter evolutionary distances [13]. Museum samples are shown by blue and red (for type specimens) dots. Collection dates are shown in parentheses.

Meusnier et al. BMC Genomics 2008 9:214   doi:10.1186/1471-2164-9-214
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