Research article
ERK1 and ERK2 MAPK are key regulators of distinct gene sets in zebrafish embryogenesis
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* Corresponding authors: SF Gabby Krens S.F.G.Krens@biology.leidenuniv.nl - Herman P Spaink H.P.Spaink@biology.leidenuniv.nl
Institute of Biology, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands
BMC Genomics 2008, 9:196 doi:10.1186/1471-2164-9-196
Published: 28 April 2008Additional files
Additional file 1:
Additional data is submitted as tables S1 to S6 consists of the ERK1 and ERK2 knockdown commonly and anti-correlated regulated probes (table S1 to S4), containing the assigned gene designations (Unigene, accession number and sequence name), the fold of the changed expression and p-value (smaller than 10-5 to compensate for multiple testing false positives) for these genes. The tables S5 and S6 contain genes selected by a stringent selected that were only found in either ERK1MO or ERK2MO gene-pools were manually annotated and assigned gene designations as listed in. Table S1 – Anti-correlated regulated genes1: ERK1MO up-regulated, ERK2MO down-regulated. Table S2 – Anti-correlated regulated genes2: ERK1MO down-regulated, ERK2MO up-regulated. Table S3 – Commonly down-regulated genes by ERK1or ERK2 knockdown at 30% epiboly. Table S4 – Commonly up-regulated genes by ERK1or ERK2 knockdown at 30% epiboly. Table S5 – ERK1 knockdown specific genes at 30% epiboly, filtered by a 1.5 fold up- or down- regulation per experiment and a common P-value of 10-5 . Table S6 – ERK2 knockdown specific genes at 30% epiboly, filtered by a 1.5 fold up- or down- regulation per experiment and a common P-value of 10-5
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Additional file 2:
ERK1 knockdown phenotype at 24 and 48hpf. Images show representative examples of surviving ERK1 morpholino injected embryos with a tailless phenotype at 24 and 48hpf.
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